β-actin mouse primer 序列 β-actin(β-肌动蛋白)是一种高度保守的紧张蛋白,在多种细胞类型中广泛表达,具有重要的细胞骨架结构和功能。由于其表达水平稳定且在多种实验研究中被广泛应用,β-actin的引物序列和扩增方法成为了常用的内参控制方法之一。以下是β-actin鼠引物的序列: 前向引物:5'-AGCCATGTACGTAGCC...
β-actin mouse primer序列-回复 在分子生物学和基因表达研究中,基因的测定和定量是非常重要的。为了确定一个基因的表达水平,研究人员通常使用RT-PCR(逆转录聚合酶链反应)技术来扩增目标基因的片段,并对扩增的产物进行测定和定量分析。在RT-PCR中,引物是扩增过程中的关键因素之一。β丝氨酸(β-actin)是一种常用的...
Beta-actin(β-actin)是一种细胞骨架蛋白质,在细胞的结构和功能中起着重要作用。β-actin在多个细胞类型中广泛表达,并且其表达水平相对稳定。因此,β-actin常用作实时定量PCR(qPCR)或RT-qPCR的内参基因,来校正不同样本之间的变异。 要使用β-actin小鼠引物序列进行PCR实验,首先需要设计适当的引物。设计引物时,需要考...
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英文名称:Actin, beta, Mouse, Primer Pair 英文同义词:Actin, beta, Mouse, Primer Pair CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览Actin, beta, Mouse, Primer Pair国外供应商中国供应商 Actin, beta, Mouse, Primer Pair推荐供应商 ...
PCR of genomic DNA was performed using Q5 DNA polymerase (NEB) and primers (Supplementary Data 2) designed against an amplicon of 150–600 bp targeting the CRISPR-locus or the CRISPOR-predicted off-targets19 (Table S1). First-round PCR primers contained adapter sequence for DNA/RNA UD In...
s were designed according to the general guidelines outlined in the Primer Express®User Bulletin. Details of the primers and the Genebank accession numbers are given in Table1. The designed primers shared 100% homology with the target sequence but no significant homology with other sequences....
A differential display polymerase chain reaction (DDPCR) protocol was used to amplify segments of sequence from the cDNA samples. Three 10-mer primers DM1 (ATATCTGGAG), DM2 (CGATCGTGCA), and DM4 (CGGTAACAAG) were used separately, each in combination with an equimolar mixture of clamped oligo...
Primers for real-time PCR in this study are as shown in Supplementary information, Table S2. ELISA To determine the secretion of human MMP1, cell culture supernatants were collected after 48 h of culture. The levels of human MMP1 protein were measured by a Human MMP1 ELISA Kit (Cusabio...
The primer sequences for each gene were as follows: HAdV-2 E1A, 5′-GTGCCAGCGAGTAGA-3′ and 5′-GTCAGAAAACCTGGT-3′; HAdV-2 L3, 5′-CAGGATGCCTCGGAGTACCTGAG-3′ and 5′-GGAGCCACGGTGGGGTT-3′; Actin, 5′-CTGGGACGACATGGAGAAAA-3′ and 5′-AAGGAAGGCTGGAAGAGTGC-3′. The...