The mammalian brain contains many specialized cells that develop from a thin sheet of neuroepithelial progenitor cells. Single-cell transcriptomics revealed hundreds of molecularly diverse cell types in the nervous system, but the lineage relationships b
(A,B) Inverse correlation between miR-125a-3p and Fyn protein during maturation: RNA (30 oocytes at each experimental group) and protein (75 oocytes at each experimental group) were extracted from GV, GVBD and in-vivo matured MII oocytes. Whole cell lysates were subjected to (A) qPCR ...
Apolipoprotein B (dilution 1:1,000) Aalto Bio Reagents Cat#BB2285 Calreticulin (dilution 1:1,000) Cell Signaling Technology Cat#122238; RRID: AB_2688013 CD9 (dilution 1:1,000) System Biosciences Cat#ExoAB-CD9A-1; RRID: AB_2687469 GM130 (dilution 1:1,000) Abcam Cat#ab52649; RRID: ...
The top line chart and the boxplot under show the number of single-cell clones with a specific mutation type, i.e., number of clones, and the percentage distribution of a specific mutation type in all single-cell clones, i.e., proportion (%), respectively. B–E Representative target ...
Purified Con A receptor glycoproteins from the P815 cell line Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. Concentration 0.5 mg/ml...
T-cell hybridoma assay 1× 105fresh responder 3A9 cells were cocultured with 2 × 105APC-preincubated 3A9 cells in 96 flat-bottom micrometer plates. After 24 hours 50 μL supernatant was harvested and IL-2 activity determined by addition to the IL-2–dependent CTLL-2 cell line. CTLL-2...
Fig. 1: Clonal relationships of forebrain cell classes determined via simultaneous capture of transcriptome and lineage barcodes from single cells. a, Schematic of STICR experimental workflow.b, Images of coronal brain sections injected with STICR.c, Quantification of the average clone size. The er...
Nature Cell Biology22,651–662 (2020)Cite this article 8054Accesses 21Altmetric Metrics Abstract BMP4 regulates a plethora of developmental processes, including the dorsal–ventral axis and neural patterning. Here, we report that BMP4 reconfigures the nuclear architecture during the primed-to-naive ...
SUPeR-seq analysis of a single HEK293T cell covered 30 % of these potential poly(A)- RNAs, and the coverage was over 50 % with the ensemble of seven individual cells (Fig. 1b). We also checked the coverage on histone RNAs [21], and found that SUPeR-seq recovered more poly(A)- ...
6B. Apparently, this antibody detected a specific band of Acdp1 in all cell lysates. Of note, shown in Fig. 6B are signals of 10 μg extracts of HEK293 cell lysates, 100 μg extracts of 3T3 and PC12 cell lysates. Thus, the expression levels of Acdp1 in these cell types vary a ...