immunizedmouse. The fusion of a myeloma cell from a line that has lost the ability to secrete immunoglobulin with a B cell known to secrete a particular antibody results in a remarkable hybrid cell that produces the antibody made by its B-cell component but retains the capacity of its ...
Sample: Jurkat human acute T cell leukemia cell line Western Blot 1-2 µg/mL Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, NIH‑3T3 mouse embryonic fibroblast cell line, Rat‑2 rat embryonic ...
Species Specificity: human, mouse, rat, yeast, plant, zebrafish Cited Species: Arabidopsis, Bombyx mori, bovine, Branchiostoma belcheri, brovine, Caenorhabditis elegans, canine, chicken, CHO cell line, Cynomorium songaricum 抗体保存 -20℃保存,不建议分装保存。
Cultures were prepared from post-natal NMRI BomTac mouse pups (Taconic) at 1–2 days of age. Whole brains were trypsin digested and made into a cell suspension. Cells were seeded in flasks pre-coated with 0.1 mg/ml poly-L-lysine (Sigma P1399) and maintained in DMEM supplemented with...
Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, ...
cell manipulations and culturing; H.L., R.B., A.C. and T.B.-G. performed molecular biology and genotyped all ES cell lines and mice; S.C. microinjected ES cells into blastocysts and maintained the mouse colonies; D.S. and B.F. analyzed mouse immune cells by FACS; A.W. and H....
A major stumbling block was the fact that the production of early monoclonal antibodies was limited by whether or not there was a suitable myeloma cell line available (usually mouse or rat). Hybridomas may also be low yielding or genetically unstable [6]. More recently, many different ...
CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, ...
TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are...
Detection of Mouse Semaphorin 5A by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Semaphorin 5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5896) followed by HRP...