Surface proteins used for subsequent flow cytometry analysis are indicated to the right. d Correlation analysis (spearman’s rho) comparing autoCSC and RNA-seq with flow cytometry. e, f Box-plots showing autoCSC obtained surface abundance values for (e) CD20 and (f) CD180 in B cell ...
Using a method which allows simultaneous flow cytometric detection of cell surface markers and 5-bromo-2'-deoxyuridine (BrdU) incorporation, the distribution and proliferative behavior of B lineage subpopulations was studied in intact adult mice. In the bone marrow we could define two subsets of B...
Cell surface markers of mouse thymic dendritic cells have been studied by flow cytometry after isolation by collagenase digestion, separation of the low-density cell fraction and differential adherence. The dendritic cell preparation had a purity of > 90%, the contaminating population being essentially...
Cell surface markers of mouse thymic dendritic cells have been studied by flow cytometry after isolation by collagenase digestion, separation of the low-density cell fraction and differential adherence. The dendritic cell preparation had a purity of > 90%, the contaminating population being essentially...
Flow cytometric estimation of LDL receptor (LDLR) expression, along with associated B cell markers, was also conducted. Kit based estimation of serum IgG, western blotting for LDLR estimation on total splenocytes and spectrometry for cholesterol and serum protein estimation were further undertaken. ...
map analysis revealed a high degree of overlap between mouse and human neutrophils, while cell distribution was driven predominantly by experimental conditions (Fig.6d). Correspondingly, activated neutrophils of both species displayed a continuous upregulation of the inflammatory response markers (Fig.6e)...
Viability assessments (post-thaw) Viable cell count % Viability Drug resistance characterization Test for antibiotic resistance High quality Gibco MEFs support healthy PSC cultures that form compact colonies with defined edges and express pluripotency marker...
Diagnostic markers Stroke CNS cancer Techniques Flow Cytometry Tissue Digestion FACS RNA Extraction Homogenization Immunostaining Immunolabeling Transcriptomics Proteomics Cell And Tissue Culture Dissection Cell Lysis Real-time PCR RNA-Seq Analysis Electrophoresis ...
流式细胞分析 (Flow) 1-10 µg/mL 产品规格 种属反应 Mouse 宿主/亚型 Goat / IgG 分类 Polyclonal 类型 Secondary Antibody 抗原 Gamma Immunoglobins Heavy and Light chains 偶联物 APC Request custom conjugation 激发/发射光谱 651/660 nm查看光谱 ...
Diagnostic markers Flow cytometry Stroke This article is cited by Profiling human brain vascular cells using single-cell transcriptomics and organoids Elizabeth E. Crouch Loukas N. Diafos Eric J. Huang Nature Protocols (2024) Regulation of the blood-brain barrier function by peripheral cues in ...