Flow Cytometry Tools for Experimental Design Flow Cytometry Panel Builder Tool Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool. Build Your Panel Interactive Cell Marker Tool Learn more about cell surface and intracellular markers from R&D Systems,...
Flow cytometry has been conventionally associated with the use of monoclonal antibodies to identify immuno-competent cells, to quantify changes in expression of surface determinants, and to separate cell population subsets before testing their functional characteristics. Multi-laser system, developments of ...
The microspheres function as reproducible size markers (Figure 4) and can be intermixed with the experimental sample or used in parallel runs. Features include: Non-fluorescent beads 1–15 µm range Read the Flow Cytometry Size Calibration Kit product ...
Using a method which allows simultaneous flow cytometric detection of cell surface markers and 5-bromo-2'-deoxyuridine (BrdU) incorporation, the distribution and proliferative behavior of B lineage subpopulations was studied in intact adult mice. In the bone marrow we could define two subsets of B...
A comprehensive suite of trusted products, integrated solutions, useful tools and a wealth of information to advance your flow cytometry applications. Companion Diagnostics Learn More Panel Design Learn More BD CellView™ Image Technology Learn More Single-Cell Multiomics Learn More Designing...
Here we describe the procedures to quantify and/or purify various B fractions in mouse bone marrows by flow cytometry using their signature surface markers. This method is useful to study B-cell development during steady-state or emergency hematopoiesis such as viral infections....
Learn more about basics of panel design and flow cytometry controls. Beads are recommended when: Multiple fluorophore emissions overlap in the same detector (Figure 1) Poorly expressed markers do not express a large distinction between po...
B cells with high side scatter parameter by flow cytometry correlate with inferior survival in diffuse large B-cell lymphoma. B cells with high side scatter parameter by flow cytometry correlate with inferior survival in diffuse large b-cell lymphoma. Am J Clin Pathol. 2012; ... A Bashashati...
The recent active use of immunotherapy based on CD19/CD3 bispecific T-cell engager (blinatumomab), brings significant problems on monitoring Minimal Residual Disease (MRD) based on Multiparameter Flow Cytometry (MFC), due to possible CD19 downregulation by tumor cells. This phenomenon breaks the we...
Flow Cytometry in the Clinical Laboratory The use of flow cytometry to analyze cell populations has expanded rapidly in recent years. Flow cytometry has proven to be a valuable research tool in the study of leukemias, autoimmunity, cell cycle kinetics, and chromosomal markers. O... KV Waller,DB...