Plasma cell dyscrasiasFlow cytometryImmunophenotyping is being routinely used for the diagnosis of leukemias. With the advent of specific markers for plasma cells, it has become possible to differentiate between benign and malignant plasma cells based on their immunophenotypic profile. The enhanced use ...
Optimisation of flow cytometry for the investigation of plasma cell dyscrasias Australian Journal of Medical ScienceIrwin, RebeccaLemmert, KarlaVanniasinkam, Thiru
Exception in myeloma with t(11:14) where plasma cells more often express B cell markers includingCD19andCD20 Flow cytometry images Contributed by Genevieve M. Crane, M.D., Ph.D. Dim kappa Strong cytoplasmic expression Neoplastic plasma cells Myeloma with t(11;14) and partial CD19 Images hos...
摘要: SummaryIntroduction: Flow cytometry (FC) has become increasingly utilized in the diagnosis and monitoring of plasma cell myeloma (PCM), though few studies have evaluated the longitudinal stability of antigen expression.DOI: 10.1111/j.1751-553x.2011.01317.x 年份: 2011 ...
(D)J sequencing. We applied a graph-based clustering algorithm to identify nine clusters, which were organized into transitional B cells, mature B cells, memory B cells, plasmablasts (PBs), and PCs (Fig.4A), based on the expression of B cell lineage markers includingCd24a,Fcer2a,Ccr6,...
Flow cytometry is also useful in the diagnosis of plasma cell dyscrasias. Plasma cells areCD38+(bright) and CD138+(although less sensitive). Plasma cells in the blood andtonsilsare CD45+, whereas in the bone marrow plasma cells may be either CD45+positive or CD45−. Normal plasma cells...
ii) the analysis of potentially aberrant phenotypic markers and to report the antigen expression pattern on neoplastic plasma cells as being reduced, normal or increased, when compared to a normal reference plasma cell immunophenotype (obtained using the same instrument and parameters); and iii) the...
In cases in which 6-color flow cytometric analysis improved detection of a clonal population, concurrent biopsy showed less than 5% involvement by plasma cell myeloma, suggesting that 6-color flow cytometry has an advantage in patients with a low disease burden. In addition, the simplification of...
Plots were generated using FlowJo software (Online Methods). During sorting, surface marker expression for additional markers in the Euroflow panel (CD19, CD81, CD27, CD56, CD117, CD45) was recorded for each single cell (Online Methods). Extended Data Fig. 2 Quality control metrics of ...
Cell–cell and cell–matrix interaction Pathogen–host interaction Seminal plasma protein expression was analysed in patients with primary and secondary infertility who presented high levels of oxidative stress (ROS+; ⩾20 RLU/s/10 million spermatozoa) versus those with physiological levels of oxida...