Protocol to obtain high-quality single-cell RNA-sequencing data from mouse liver cells using centrifugationSimeng Wang Person Envelope
For bulk cell RNAseq, single-cell suspensions of day 5 thymi of BALB/cByJ mice were stained with anti-TCRγδ (GL3) and other surface markers and sorted using a Moflo-XDP (Beckman Coulter). TRIzol (Life Technologies)-chloroform (Sigma) extraction protocol was used to isolate RNA obtained...
The mechanisms of radiation-induced liver injury (RILI) has not been fully elucidated so far. In the present study, a RILI mouse model was constructed by exposing the liver to a single dose of 30 Gy X-rays. Liver injuries c
cell depletion, or juvenile animals where not all stages of spermatogenesis have been completed. We present single-cell RNA sequencing (scRNA-Seq) data of 2,500 cells from the tes
study, we combined flow cytometry and single-cell RNA sequencing (scRNA-seq) technology to reveal the heterogeneity of MoMF and their functional changes after MSC treatment on CCl4-induced ALI mouse model, with the hope to reveal the underlying mechanism for MSC to treat ALI through liver ...
Abcam ab18102 Mouse monoclonal [2C1.3A11] to Nestin - Neural Stem Cell Marker 100ul 3996 Nestin 抗体 一抗 Hu Ms 2C1.3A11 IgG1 FC,ICC,IP,WB Abcam ab18103 Mouse monoclonal [149/1] to Activin Receptor Type IIA - BSA and Azide free 100ug 3996 Activin Receptor type II 抗体 一抗 Hu ...
1、小鼠肝成纤维细胞MouseLiver:NormalLiverFibroblasts所有的细胞表面均有由磷脂双分子层与镶嵌蛋白质及糖被构成的生物膜,即细胞膜。 2、所有的细胞都含有两种核酸:即DNA与RNA。 3、作为遗传信息复制与转录的载体。 4、作为蛋白质合成的机器—核糖体,毫无例外地存在于一切细胞内,核糖体,是蛋白质合成的机器,在细胞...
This work provides a crucial resource to resolve molecular and cellular information in normal, diseased, and developing human and mouse livers. 展开 关键词: analysis pipelines cellular heterogeneity database liver cells single cell RNA-seq DOI: 10.1002/smtd.202201421 年份: 2023 ...
(Supplementary Fig.1e). We evaluated binding of the HARD protein to various nucleic acids using isothermal titration calorimetry (ITC). The HARD protein bound to 8nt single-stranded RNA, double-stranded RNA, single-stranded DNA, and double-stranded DNA with around 1 µM affinities (Fig.1...
The rapidly labeled RNA from both the nuclei and cytoplasm of mouse liver cells can be bound specifically to mouse DNA. The bound fraction differs in base composition and metabolic stability from the bulk RNA. There is considerable cross reaction between this RNA and the DNA obtained from calf ...