5) monocyte-derived macrophages 单核源巨噬细胞 1. Objective To investigate the clinical significance of matrix metalloproteinase-9 (MMP-9) and tissue factor (TF) secreted by cultured monocyte-derived macrophages (HMDM) from patients with coronary heart disease (CHD) in vitro, and to evaluate ...
Whether primary monocyte-derived macrophages (MDMs) support WNV infection-replication and produce infectious virions, with an in vitro system, was investigated. Study design and methods:Elutriated monocytes (CD33+/CD14+) from suitable blood donors were cultured in the presence of macrophage-colony-...
1.Objective To investigate the clinical significance of matrix metalloproteinase-9 (MMP-9) and tissue factor (TF) secreted by culturedmonocyte-derived macrophages(HMDM) from patients with coronary heart disease (CHD) in vitro, and to evaluate the intervenient effect of puerarin (Pur) on them.目...
Clinical and experimental research has shown that reduced levels of brain-derived neurotrophic factor (BDNF) may be a risk factor for neurological complications associated with HIV-1 infection. We are actively testing genetically modified macrophages for their possible use as the cell-based gene ...
Transcriptional profiling of macrophages derived from monocytes and iPS cells identifies a conserved response to LPS and novel alternative transcription. Sci Rep. 2015;5(1):12524. doi:10.1038/srep12524.Alasoo K, Martinez FO, Hale C, Gordon S, Powrie F, Dougan G, Mukhopadhyay S, Gaffney DJ....
Monocyte-derived macrophages (MDM) were differentiated in vitro in the presence of TMVs obtained from colon cancer: Caco-2, SW620, LoVo or SW480 cell lines and analysed according to their morphology and biological functions, as defined by cytokine secretion, reactive oxygen intermediate (ROI) prod...
Alveolar macrophagesCD14In vitro induction of maturation of blood monocytes by 5 day culture of peripheral blood mononuclear cells in 10 % human serum will result in decreased CD14 and increased CD16 cell surface expression on the monocyte derived macrophages. At the same time, these cells acquire...
leprae-infected CD4(+) T cells, monocytes, and monocyte-derived macrophages. Data revealed that both protein and mRNA levels of IRGM were increased in monocytes after M. leprae infection. Interestingly, monocyte-derived macrophages showed more prominent IRGM expression with M. leprae infection, ...
Our understanding of the ontogeny of monocytes and macrophages, as well as their maintenance in the steady state, has recently undergone a renaissance. Here, Ginhoux and Jung discuss the evidence that has changed our view of the relationship between mono
The RNA editing in monocytes is activated by hypoxia and IFNs in both independent and additive manners (Figs 1a and 2a), and in monocyte-derived macrophages by M1 but not M2 polarization (Figs 1b and 2a). These findings represent the discovery of the first mammalian RNA-editing CDA enzyme...