MDMs obtained in the culture condition herein described represent a valuable model to disentangle and manipulate the functional heterogeneity of tissue macrophages that has been disclosed in scenarios spanning from inflammatory and wounding responses to atherosclerotic lesions. J. Cell. Physiol. 228: ...
Macrophages from coronary plaques are not available, and monocyte-derived macrophages (MDMs) are usually considered as a surrogate. We compared the MDM profile obtained from coronary artery disease (CAD) patients and healthy subjects, and we evaluated the association between CAD MDM profile and...
Several lines of evidence show that human immunodeficiency virus (HIV) persists in blood monocytes and tissue macrophages in virologically suppressed people with HIV (vsPWH)1. HIV DNA has been detected in highly purified monocytes1,2,3,4,5and macrophages isolated from the urethra6, gut7, liver...
The success of each VOC, as well as the virus fitness, were enabled by altered intrinsic functional properties and, reasonably, to virus antigenicity changes, conferring the ability to evade a primed immune response.#We analysed the gene expression profiles of monocyte-derived macrophages (MDM) ...
Monocyte-derived macrophages (MDMs) exhibit a high degree of functional plasticity. Here, we determined the role of MDMs in long-term spontaneous functional recovery after middle cerebral artery occlusion in mice. Analyses by flow cytometry and immunocytochemistry revealed that monocytes home to the ...
HIV-1 isolates were used to infect monocyte-derived macrophages (MDMs) and infection monitored by secreted HIV-1 p24 by enzyme-linked immunosorbent assay (ELISA). Cell lysates of uninfected and HIV-1–infected MDMs at 14 days post infection were fractionated by cationic exchange chromatography and...
Here, we investigated IL-10 expression in primary cultures of monocyte-derived macrophages (MDMs) in response to in vitro infection by HIV-1/Ba-L or two macrophage-tropic HIV-1 primary isolates. Whatever the multiplicity of infection used, and in spite of high replication levels and an ...
Figure 1. Comparison of cell surface molecule expression of alveolar MΦ (AM) and GM-CSF or M-CSF-derived MDMs (monocyte-derived macrophages). Primary human monocytes from healthy donors were differentiated into MDMs in vitro in the presence of GM-CSF (GM-MDMs) or M-CSF (M-MDMs) for 6...
Infected ciliated airway epithelial cells, local macrophages and dendritic cells secrete cytokines including interleukins (IL) 6 and 8, promoting a strong neutrophilic response that is important in disease clearance and airway inflammation. In vitro, RSV is capable of infecting MoDCs. RSV inhibits ...
2010; In vitro response of monocyte-derived macrophages to a decellularized pericar- dial biomaterial. J Biomed Mater Res A 93A(1): 280-288.Ariganello MB, Labow RS, Lee JM. In vitro response of monocyte-derived macrophages to a decellularized pericardial biomaterial. J Biomed Mater Res A ...