We report the induction of IRF-7 expression by 12-O-tetradecanoylphorbol-13-acetate in U937 and HL60 cells and demonstrate that this induction is essential for the monocyte differentiation to macrophages. We sho
Chromatin undergoes extensive reprogramming during immune cell differentiation. Here we report the repression of controlled histone H3 amino terminus proteolytic cleavage (H3ΔN) during monocyte-to-macrophage development. This abundant histone mark in human peripheral blood monocytes is catalyzed by neutrophil...
macrophage differentiationAbstract We have been interested in the immunomodulatory effect, to promote differentiation of the human monocytic cell line THP-1 to macrophages of EM-A. We chemically modified EM-A in order to obtain derivatives with stronger promoting activity of monocyte to macrophage ...
To determine this, we measured several key cell phenotypes and the activation of underlying cell signalling pathways in SVEP1 knockout (KO) monocytic cell lines undergoing differentiation to macrophages. 2. Materials and methods Reagent or resourceSourceIdentifier Antibodies Phospho-paxillin (Y118) Cell...
serum﹊nduced differentiationHLA class II antigensinterferonCells of the mononuclear phagocyte system arise from circulating blood monocytes. Upon emigration from the vasculature, monocytes differentiate into macrophages, a process that monocytes similarly undergo in vitro. We have established primary cultures...
Theurl, I. et al. On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver.Nat. Med.22, 945–951 (2016). ArticleCASPubMedPubMed CentralGoogle Scholar Menezes, S. et al. The heterogeneity of Ly6Chimonocytes controls their differentiation into iNOS+macrophages...
Macrophages, originating from the migration and differentiation of circulating monocytes into virtually all tissues, are extremely flexible and plastic cells that play vital homeostatic roles, but also contribute to the pathophysiology of many diseases. For these reasons, they are intensively studied by ...
内容提示: Unlike PPARc, PPARa or PPARb/d activation does not promote humanmonocyte differentiation toward alternative macrophagesMohamed Amine Bouhlela,b,c,d, John Brozeke, Bruno Derudasa,b,c,d, Christophe Zawadzkif, Brigitte Judef,Bart Staelsa,b,c,d,*, Giulia Chinetti-Gbaguidia,b,c,da...
A hallmark of inflammatory diseases is the excessive recruitment and influx of monocytes to sites of tissue damage and their ensuing differentiation into macrophages. Numerous stimuli are known to induce transcriptional changes associated with macrophage
The P. citrinopileatus polysaccharide (PCPS) suppresses production of pro-inflammatory cytokines in THP-1–derived macrophages stimulated with LPS/IFNγ To assess whether the P. citrinopileatus polysaccharide (glucan) PCPS modulates macrophage differentiation, we determined the levels of cytokines secreted...