Mittar, D., Paramban, R., McIntyre, C., 2011. Flow Cytometry and High-Content Imaging to Identify Markers of Monocyte-Macrophage Differentiation, BD Biosciences, Application Note, pp. 1-20.Mittar, D., Paramban, R. and McIntyre, C. (2011). Flow cytometry and high-content im...
Flow cytometry was performed on a BD LSRFortessa (BD Biosciences). Voltage settings were standardized to daily CS&T Research Bead (BD Biosciences) controls using predetermined application settings in FACSDiva 6.2 to ensure that fluorescent intensity was consistent longitudinally. Data were analysed using...
For study of monocyte surface markers with flow cytometry, a 0.4-ml aliquot was added to a pyrogen-free tube contain- ing citrate phosphate dextrose (acid citrate dextrose; Baxter Health Care, Norfolk, UK) and was immediately cooled to 0 °C and kept until analysis in an ice-water bath...
To establish the fibrosis model, the HELF cell line was selected and was induced with TGFβ1, followed by assessment of the expression of α-SMA and collagen type I as the markers of fibrosis. The mRNA expression of α-SMA and collagen type I was found to be upregulated in the model ...
Heterogeneity among human monocytes was recognized about thirty years ago [4, 5], and several markers like CD64 [6] or CD16 [7] have been suggested for differentiating subpopulations of monocytes. The differential expression of CD14 (part of the receptor for lipopolysaccharide) and CD16 (also ...
Evaluation of tumour necrosis factor alpha, interleukin-2 soluble receptor, nitric oxide metabolites, and lipids as inflammatory markers in type 2 diabetes mellitus. Mediators Inflamm. 2006;2006(1):39062. doi:10.1155/MI/2006/39062. PubMed PubMed Central Google Scholar Bano G. Glucose ...
CD41 were measured by flow cytometry.Results The expression levels of CD14,CD16 and CD41 on monocyte in group of cerebral infarction patients were significant difference than those in normal control(P0.001).Conclusion The monocyte and platelet activity both increases in patients with cerebral ...
Thomas, G. D., et al.Human Blood Monocyte Subsets: A New Gating Strategy Defined Using Cell Surface Markers Identified by Mass Cytometry.Arteriosclerosis, Thrombosis, and Vascular Biology.37(8), 1548-1558 (2017). Tags Monocyte SubsetsFlow CytometryWhole Blood AnalysisCD14CD16HLA-DRM1 And...
(a) Western blot and real-time PCR analyses of expression of ZFP36L1 and the monocyte/macrophage differentiation markers CD11B, CD14 and CSF1R. THP-1 cells were infected with lenti-shZFP36L1 and lenti-ctrl respectively followed by PMA induction for 50 h. (b) Expression of the monocytic...
2b). After 48 h in culture with M-CSF, cMoP could no longer be distinguished in flow cytometry from monocyte-derived macrophages with respect to the typical MΦ-surface markers CD11b and F4/80 (Fig. 3b). Fig. 3: cMoP-derived MΦ keep their potential to form MGC and show a ...