Mittar, D., Paramban, R., McIntyre, C., 2011. Flow Cytometry and High-Content Imaging to Identify Markers of Monocyte-Macrophage Differentiation, BD Biosciences, Application Note, pp. 1-20.Mittar, D., Paramban, R., Mcintyre, C. (2011). Flow cytometry and high-content imagin...
There is also substantial overlap among signature markers for M1 and M2, representing a potential continuum of phenotypes. Additionally, as M1 and M2 signature markers are shared between microglia and macrophages in the stroked hemisphere, future studies should characterize the functions for each ...
To establish the fibrosis model, the HELF cell line was selected and was induced with TGFβ1, followed by assessment of the expression of α-SMA and collagen type I as the markers of fibrosis. The mRNA expression of α-SMA and collagen type I was found to be upregulated in the model ...
All selected monocyte samples were analysed by flow cytometry to determine the percentage of contaminating T cells before plating. Once plated, the cells were cultured in the presence of ART for 7 d for further purification by adherence. Once the macrophages were differentiated, they were washed ...
(a) Western blot and real-time PCR analyses of expression of ZFP36L1 and the monocyte/macrophage differentiation markers CD11B, CD14 and CSF1R. THP-1 cells were infected with lenti-shZFP36L1 and lenti-ctrl respectively followed by PMA induction for 50 h. (b) Expression of the monocytic...
The phenotype of the DCs was determined by labeling surface markers and analyzing them using flow cytometry. Afterward, real-time PCR was carried out to evaluate the expression of inflammatory and anti-inflammatory genes. The administration of RAD001 to DCs led to a significant upregulation in ...
Heterogeneity among human monocytes was recognized about thirty years ago [4, 5], and several markers like CD64 [6] or CD16 [7] have been suggested for differentiating subpopulations of monocytes. The differential expression of CD14 (part of the receptor for lipopolysaccharide) and CD16 (also ...
(C) Flow cytometry mean fluorescence intensity (MFI) for exhaustion (CD38, MARCO, PD-L1, CXCR2) and macrophage (F4/80) markers relative to PBS control. Boxplots indicate median MFI values (n = 5–7; one-way ANOVA with Sidak’s multiple comparisons test). (D) Gating strategy (top) ...
Similarly, depletion of rLCs using a single injection of DT before injecting IL-23 had little effect on the mediators and markers of inflammation. In contrast, persistent elimination of all langerin þ cells by repeated injections of DT, including not only the langerin þ dermal cDCs but ...
5a,b). In contrast, the expression of Ym1, a well-established M2 marker, was less in Angptl4−/− BMDMs after M2 polarization, while the expression of other M2 markers was not changed (Supplementary Fig. 5a). Monocyte/macrophage migration is an important step in the early atherogenic ...