Mittar, D., Paramban, R., McIntyre, C., 2011. Flow Cytometry and High-Content Imaging to Identify Markers of Monocyte-Macrophage Differentiation, BD Biosciences, Application Note, pp. 1-20.Mittar, D., Paramban, R., Mcintyre, C. (2011). Flow cytometry and high-content imagin...
Fig. 1.Origins and expression of cell surface markers by monocytes and macrophages. Monocytes and macrophages have a common origin in hematopoietic stem cells (HPSCs). Hematopoietic stem cells are multipotent stem cells that reside in the bone marrow. In the presence of colony-stimulating factor 1...
Thus, stable CAD is associated with expansion of the nonclassical monocyte subset and increased expression of inflammatory markers on monocytes. Flow cytometric analysis of monocyte subsets and marker expression may provide valuable information on vascular inflammation. This may translate into the ...
To use cell markers as predictors of disease requires that they be measured reliably and show modest within-individual, day-today variation. METHODS: We used whole blood flow cytometry to analyze monocyte and platelet markers in the Atherosclerosis Risk in Communities (ARIC) Carotid MRI study. We...
Massanella, M. et al. Long-term effects of early antiretroviral initiation on HIV reservoir markers: a longitudinal analysis of the MERLIN clinical study.Lancet Microbe2, e198–e209 (2021). ArticleCASPubMedPubMed CentralGoogle Scholar McNamara, L. A. et al. CD133+ hematopoietic progenitor cell...
Carotid stenosis (CS) is an important cause of ischemic stroke. However, reliable markers for the purpose of identification of high-risk, so-called vulnerable carotid plaques, are still lacking. Monocyte subsets are crucial players in atherosclerosis and might also contribute to plaque rupture. In ...
3. Nested scatterplots of flow cytometry performed on samples from n = 3 donors. HLA-DR mean fluorescence intensity (MFI), fraction of live cells expressing dendritic cell differentiation markers CD11c + CD1c + , CD80 + , and PDL1 + gated on live-HLA-DR + ...
To annotate cell types, differentially expressed genes (DEGs) among different groups were identified using the FindAllmarkers function with the Wilcox test approach. The parameters used for this analysis were as follows: only.pos = TRUE, min.pct = 0.2, logfc.threshold = 0.25. ...
In general, breast cancer is divided into 5 subtypes based on the expression or absence of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her2) and proliferation markers. ER positive (ER+) tumors are the most prevalent subtype with 70–75% ...
Compared with the lenti-ctrl-infected THP-1 cells, the lenti-ZFP36L1-infected cells exhibited significant over-expression of ZFP36L1 which increased mRNA levels of the monocyte/mac- rophage differentiation markers (CD11B, CD14 and CSF1R) (Fig. 3d). Besides, the over-presence of ZFP36L1...