Mittar, D., Paramban, R., McIntyre, C., 2011. Flow Cytometry and High-Content Imaging to Identify Markers of Monocyte-Macrophage Differentiation, BD Biosciences, Application Note, pp. 1-20.Mittar, D., Paramban, R. and McIntyre, C. (2011). Flow cytometry and high-content im...
For study of monocyte surface markers with flow cytometry, a 0.4-ml aliquot was added to a pyrogen-free tube contain- ing citrate phosphate dextrose (acid citrate dextrose; Baxter Health Care, Norfolk, UK) and was immediately cooled to 0 °C and kept until analysis in an ice-water bath...
9 RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook M/M (redirected fromMonocyte/Macrophage Lineage) AcronymDefinition M/MMr. and Mrs. M/MMulti-Media M/MMale/Male M/MMan/Machine M/MMonocyte/Macrophage Lineage(immunology) ...
To use cell markers as predictors of disease requires that they be measured reliably and show modest within-individual, day-today variation. METHODS: We used whole blood flow cytometry to analyze monocyte and platelet markers in the Atherosclerosis Risk in Communities (ARIC) Carotid MRI study. We...
To annotate cell types, differentially expressed genes (DEGs) among different groups were identified using the FindAllmarkers function with the Wilcox test approach. The parameters used for this analysis were as follows: only.pos = TRUE, min.pct = 0.2, logfc.threshold = 0.25. ...
3. Nested scatterplots of flow cytometry performed on samples from n = 3 donors. HLA-DR mean fluorescence intensity (MFI), fraction of live cells expressing dendritic cell differentiation markers CD11c + CD1c + , CD80 + , and PDL1 + gated on live-HLA-DR + ...
Flow cytometric analysis reveals that the heterogeneous phenotypes of intestinal macrophages differ from circulating Ly6Chi monocytes based on specific surface markers [20]. By combining flow cytometric analysis with principle component analysis, infiltrated monocytes are observed to fail to differentiate into...
We performed flow cytometry with antibody markers for monocytes (CD14), platelets (CD42a), and TF. We compared results of patients with HI (Glasgow Coma Scale score < or = 9 and Abbreviated Injury Scale Head/Neck score > or = 3) with patients without HI and with controls.#Patients had ...
(cytometry by time of flight–CyTOF) with visual interactive stochastic neighbor embedding techniques are currently being developed by us and others, to improve the sensitivity and specificity of flow based techniques to diagnose CMML11. Using this method it has been shown that CD14/16 markers are...
The monocyte data are reported post-adjustment for CD4 T cell contamination as measured by flow cytometry at the time of isolation (described in Extended Data Table 2 and methods). Overall, CD4 T cells had higher levels of intact, 3’ defective and 5’ defective proviruses versus monocytes. ...