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Then, the tube-like structures were fixed with 4% PFA and observed under a microscope. With respect to immune modulatory activity, primary mouse macrophage (M0) RAW264.7 cells were cultured in DMEM containing 10% FBS at 1 × 106 cells/well in 6-well plates with 10 ηg/ml LPS in the ...
The immune response to mycobacteria is characterized by granuloma formation, which features multinucleated giant cells as a unique macrophage type. We previously found that multinucleated giant cells result from Toll-like receptor-induced DNA damage and cell autonomous cell cycle modifications. However, ...
Transmigrated monocytes were counted in five arbitrary images under a microscope. 2.7. JAM-A, Cx43, and VE-Cadherin Small-Interfering RNA HUVECs were cotransfected with effectene transfection reagent (QIAGEN) that carried JAM-A, Cx43, and VE-cadherin selective small-interfering RNA (siRNA) for...
stained with Hoechst and monitored by confocal microscopy (representative ofn = 3 independent repeats).gSchematic diagram of a mouse IAV infection model. The total number of A5-positive mouse monocyteshand monocyte ApoBDsiwithin the BAL were quantified by flow cytometry under the following infect...
(Hexb) overnight at 4 °C. The sections were then incubated with the appropriate secondary antibodies. DAPI (Beyotime, China) was used to stain the cell nuclei. Images were taken with a fluorescence microscope (Olympus, Tokyo, Japan). Immunofluorescence revealed that dopaminergic neurons in ...
Images were acquired using a Nikon ECLIPSE Ti2 Inverted confocal microscope with a motorized stage and a Nikon C2 laser system (Nikon, Melville, NY). Evaluation of lesion volume and cell quantification by optical fractionator Serial coronal sections were stained with Cresyl violet acetate (Electron ...
(blue). Images were acquired with a spinning-disk confocal microscope. For each condition, the images show a top view of the unprocessed fluorescence signal of a field of view (upper right) and isosurface-processed 3D reconstructions in the side view (down left). Scale bar: 10 µm.bZ...
The production of THBS1 by myeloid cells is critical for suppressing inflammation under certain pathological conditions39,40. Our analysis of the GFP-BM mice revealed a substantial number of infiltrating BM-derived myeloid cells in aggressive CRC. This is consistent with the fact that tumor cells ...
sections were incubated sequentially with primary antibodies followed by the corresponding Alexa 405/488/568/Cy5™ secondary antibodies (1:1000 dilution, Invitrogen) and DAPI (Sigma–Aldrich; 1:1,000) staining. Z-stacks images were taken with a Leica TCS SP8 confocal microscope and 3D-analyzed ...