The fluorescence images were acquired using an epifluorescence microscope (Olympus, BX60). For ELISA experiments, the supernatants of THP1, M0 macrophages and M2 macrophages were harvested for the measurements of IL10, CCL18 and CCL22 by human ELISA kits (Human IL-10 Elisa Kit, #EH10245S, ...
While preclinical studies support their functional involvement, this study has characterized monocyte changes under neoadjuvant BC treatment and found associations of patient response with subset distribution and ER expression of monocytes. Due to the limited number of good responders when treatment arms ...
During infection, inflammatory monocytes are thought to be key for bacterial eradication, but this is hard to reconcile with the large numbers of neutrophils that are recruited for each monocyte that migrates to the afflicted tissue, and the much more ro
Images were acquired using Leica TCS SP2 AOBS (Leica Microsystems, Germany) confocal microscope or Zeiss Observer Z1 fluorescence microscope (Zeiss, Germany), respectively. Intravital microscopy. To visualize monocytes in the microcirculation the cremaster muscle of male GFP þ ctrl and GFP þ N2...
Monocytes were stained with a PE-conjugated rat anti-mouse Ly6C Ab (eBiosciences, CA, USA) and counted under the fluorescence microscope. Isolation and purification of mouse brain microvascular endothelial cells Recently, we have demonstrated that circulating BMECs (cBMECs) can be used as potential...
The cells exhibited a dose-dependent reduction in viability (Fig. 2a). Endogenous 15-oxoETE is so low that it can’t kill cells in short period. Therefore, lower doses of 15-oxoETE (0-8 μmol/L) were added to cultured HUVECs under serum deprivation, and then the cell viability was...
Tissue sections were mounted with Slow-Fade Gold Anti-Fade reagent (catalog no. S36936, Molecular Probes) and viewed with a Olympus FV500 laser scanning confocal microscope; images were captured at 400× and 600× magnification by Fluoview software Version 4.3. Statistics Wilcoxon tests were used...
The floated nuclei were counted with a hemocytometer under a phase contrast microscope to know the cell number. Culture of T3M-30Lu Cell: The human lung cancer cell line T3M-30Lu established by me, the inventor, from large cell carcinoma of the lung was cultured. The cell line had been...
Images were taken with an EVOS Fluorescence Microscope (Fisher Scientific), and the number of bound cells was manually counted with ImageJ. sEVs isolation For sEVs isolation, monocytes were plated in 90 mm cell culture dishes using a medium supplemented with exosome-free fetal bovine serum. ...
Another antibody, particularly a monoclonal antibody, of the invention has activity to induce intercellular aggregation of cells (such as cells in the monocyte/macrophage series) in the course of differentiation under stimulation by differentiation factors (such as lipopolysaccharide, PMA, cytokines, etc...