Images were visualized with a Leica DMI 6000 microscope (Wetzlar, Germany) equipped with a Micro MAX-1300YHS camera using an HCX PL APO 60X oil objective (Leica, Wetzlar, Germany). Images were captured using Metamorph Software (Version 7.1.3, Molecular Devices, San Jose, CA, USA). 2.2. ...
After staining with 4% aqueous uranyl acetate, grids were observed under a Tecnai 12 electron microscope (FEI) and digital images taken with a SIS MegaviewIII charge-coupled device camera (Olympus). 4.9. Caspase Activity FAM-DEVD-FMK labels activated caspase 3 and 7 in living cells while FAM...
Cells were imaged using the Etaluma LS720 Microscope (Carlsbad, CA, USA). Data were analysed using Image J software v1.51 for Windows. 2.5. RNA Interference Pre-designed miR-511-3p inhibitors and mimics were purchased from Qiagen, and transfection was carried out using the HiPerFect Transfection...