Cryo-EM structure of the complex between 2G1 and SARS-CoV-2 S protein To investigate the binding mode of antibody 2G1 on S trimer, we solved the cryo-electron microscopy (cryo-EM) structure of 2G1 in complex with S trimer at 2.7 Å resolution (Fig. 6a; Supplementary Figs. S6 and...
GNL = galanthus nivalis lectin, EBL = elderberry bark lectin/sambucus nigra agglutinin; HPA = helix pomatia agglutinin, VVL = vicia villosa lectin, DBA = dolichol biflorous agglutinin, A4 = mAb A4, α-Lex = anti-Lewis X antibody, α-H = anti-H type...
Neutralizing antibody titers were calculated using the Kärber formula and normalized to a 1 mg/mL solution [35]. 2.6. FPLC Gel Filtration Analysis and Immunoblotting Purified anti-PV IgA mAbs from hybridomas were dialyzed against PBS and fractionated by size exclusion chromatography (SEC) on a...