The rapid binding of 2G1 with association constant (Ka) = 2.55 × 106/Ms offered a sub-nanomolar equilibrium dissociation constant (KD) value of 0.41 nM (Fig. 2d). Next, the antibody 2G1 was moved to further characterization including in vitro and in vivo biological activities as well...
GNL = galanthus nivalis lectin, EBL = elderberry bark lectin/sambucus nigra agglutinin; HPA = helix pomatia agglutinin, VVL = vicia villosa lectin, DBA = dolichol biflorous agglutinin, A4 = mAb A4, α-Lex = anti-Lewis X antibody, α-H = anti-H type...
Neutralizing antibody titers were calculated using the Kärber formula and normalized to a 1 mg/mL solution [35]. 2.6. FPLC Gel Filtration Analysis and Immunoblotting Purified anti-PV IgA mAbs from hybridomas were dialyzed against PBS and fractionated by size exclusion chromatography (SEC) on a...
Keywords: IDH1; R132H; novel rabbit monoclonal antibody; B-cell cloning; immunohistochemistry 1. Introduction Isocitrate dehydrogenase 1 (IDH1) functions as an enzyme in the Krebs (citric acid) cycle and is biologically active in the cytoplasmic and peroxisomal compartments under normal conditions [1...