We further investigated the Fc effector function of 2G1. Results showed that 2G1 had no obvious antibody-dependent cellular cytotoxicity (ADCC) effect (not shown) but moderate antibody-dependent cellular phagocytosis (ADCP) up to 35% (Supplementary Fig. S4a). We hypothesize that the moderate ADCP...
Neutralizing antibody titers were calculated using the Kärber formula and normalized to a 1 mg/mL solution [35]. 2.6. FPLC Gel Filtration Analysis and Immunoblotting Purified anti-PV IgA mAbs from hybridomas were dialyzed against PBS and fractionated by size exclusion chromatography (SEC) on a...