Monitoring mitophagy in mammalian cells . Methods Enzymol. 547 , 39–55 (2014).Monitoring mitophagy in mammalian cells. Y. Zhu,G. Chen,L. Chen,W. Zhang,D. Feng,L. Liu,Q. Chen. Methods in Enzymology . 2014Zhu Y,
Because of their central role in many biological processes and pathological issues, mitochondria have been the focus of many investigations aiming to find evidence of selective autophagy of mitochondria, or mitophagy. Experiments on mammalian cells show that mitochondria are actively degraded after ...
Autophagy is critical not only for the cell’s adaptive response to starvation but also for cellular homeostasis, by acting as quality-control machinery for cytoplasmic components. This basal autophagic activity is particularly needed in postmitotic cells for survival maintenance. Recently, basal autophag...
Out of the three main types of autophagy coexisting in most mammalian cells — macroautophagy, microautophagy, and chaperone-mediated autophagy (CMA) — CMA is the only one solely dedicated to protein degradation3. Selectivity of CMA is conferred by a dedicated protein recognition system that ...
Gene expression in mammalian cells results from coordinated protein-driven processes guided by diverse mechanisms of regulation, including protein–protein interactions, protein localization, DNA modifications and chromatin rearrangement. Regulation of gene expression is particularly important in stress-response ...
过溶酶体膜的内陷、突起和/或分隔，直接吞入细胞浆的自噬行 为。3、分子伴侣介导的细胞自噬(chaperonemediatedautophagy，CMA)，是指由分子伴侣将靶蛋白转送至溶酶体内的自噬行为。这只见于哺乳类动物细胞。BLUKANDSELECTIVEAUTOPHAGY AUTOPHAGYINCELLULARGROWTHCONTROL AUTOPHAGYINCELLULARGROWTHCONTROL AUTOPHAGYDURINGVERTEBRATE...
Mitophagy Crosstalk between apoptosis and autophagy Crosstalk between apoptosis and autophagy Crosstalk between apoptosis and autophagy Crosstalk between apoptosis and autophagy 钙蛋白酶 Effects of Drugs on the Autophagic Pathway Effects of Drugs on the Autophagic Pathway Methods in Mammalian Autophagy Research...
A recent study has shown that in yeast cells subjected to nitrogen starvation, degradation of mitochondria by autophagy occurs by both a selective process (termed mitophagy) and a nonselective process. This chapter provides an overview of the techniques that enable the study of mitophagy. ...
In mammals, the OM contains 150 proteins, the majority of which contain α-helical transmembrane domains. This family of α-helical proteins has significantly expanded in metazoans and has evolved to mediate critical signaling and regulatory processes including mitochondrial fusion and fission, mitophagy...
Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nat. Commun. 2015, 6, 8472. [Google Scholar] [CrossRef] [PubMed] van Niel, G.; D’Angelo, G.; Raposo, G. Shedding light on the cell biology of extracellular vesicles. Nat. Rev. Mol. Cell. ...