To preliminarily explore the molecular mechanisms of tirzepatide on metabolic dysfunction-associated fatty liver disease (MAFLD), liquid chromatography-mass spectrometry (LC-MS) was employed for comprehensive metabolomic, lipidomic, and proteomic analyses in MAFLD mice fed a high-fat diet (HFD). The...
Typically, cells from 10 mL of culture were harvested and resuspended in Buffer A with 3 mL buffer for every gram of wet cell mass. Cells were directly lysed by incubation with 1% DDM for 1 h. Cell debris was removed by centrifugation at 21,000 g for 1 h, and the ...
Type 2 diabetes mellitus (T2DM) has rapidly increased worldwide, emerging as the fifth leading cause of death. The treatment of T2DM is challenging due to the side effects of oral hypoglycemic drugs and the limited efficacy of long-term insulin therapy, which can lead to insulin resistance (...
flavus contaminated maize inoculated on culture media was monitored over seven weeks and then measured using liquid chromatography–mass spectroscopy (LC-MS). Results confirmed the successful isolation of A. flavus strain VKMN22 with accession number OP355447, which consistently produced higher levels ...
This sensitivity became comparable with the sensitivity of triple quadrupole machines (24, 27), which currently are the most widely used instruments in mass spectrometric analysis of lipids (28). Collision-induced dissociation of anions of glycerophospholipids yields abundant acyl anions of fatty acid ...
Ivanova EP, Nguyen SH, Guo Y, Baulin VA, Webb HK, Truong VK, Wandiyanto JV, Garvey CJ, Mahon PJ, Mainwaring DE (2017) Bactericidal activity of self-assembled palmitic and stearic fatty acid crystals on highly ordered pyrolytic graphite. Acta Biomater 59:148–157 Article CAS PubMed Google...
HSF1 functions as a key defender against palmitic acid-induced ferroptosis in cardiomyocytes. J. Mol. Cell Cardiol. 150, 65–76 (2021). Article CAS Google Scholar Dikalova, A. E. et al. Therapeutic targeting of mitochondrial superoxide in hypertension. Circ. Res. 107, 106–116 (2010). ...
Cell. Proteomics, 2005 Annotating the Human Proteome Antibody-based Proteomics for Human Tissue Profiling Statistical and Computational Methods for Comparative Proteomic Profiling Using Liquid Chromatography-Tandem Mass Spectrometry Tissue Profiling by Mass Spectrometry Proteomics techniques and their application ...
Obesity is well-established as a significant contributor to the development of insulin resistance (IR) and diabetes, partially due to elevated plasma saturated free fatty acids like palmitic acid (PA). Grb10-interacting GYF Protein 2 (GIGYF2), an RNA-bin
[27]. Very recently, authors, carrying out a GWAS meta-analysis of imaging and diagnostic code-measured NAFLD across diverse ancestries, identified NAFLD-associated variants at torsin family 1 member B, fat mass and obesity associated, cordon-bleu WH2 repeat protein like 1/growth factor ...