分子倒置探针技术是一项新发展起来的用于目标序列捕获的分子生物学技术,该技术通过设计特异的探针对已知的特定目的基因组序列进行捕获,将目标序列DNA富集后再利用芯片杂交或测序进行检测。此技术有助于研究人员对大样本中的基因组重要区域进行研究,避免了全基因组研究费用高、分析困难等问题。并且,分子倒置探针技术弥补了杂...
Provided herein can be a method comprising forming a molecular inversion probe (MIP; a single stranded linear molecule containing two target binding arms where the arms may be separated by a backbone sequence) to selectively target nucleotide template from any source (viral, prokaryotic, and ...
To survey CNAs in childhood leukemia at the resolution of individual gene sequences, we applied a novel genomic technology (molecular inversion probes, or MIPs) that has been adapted for gene copy analysis in cancer [17], [18]. In this technology, the probe is a single oligonucleotide that ...
Here we explored the utility of Molecular Inversion Probes (MIPs) for the medium-scale targeted resequencing in a non-model system. Markers targeting 112bp of exonic sequence were designed from transcriptome ofnewts. We assessed performance of 248 MIP markers in a sample of 85 individuals. Among...
In this context, "gap-fill" molecular inversion probes, when combined with NGS, allow for the capture and evaluation of substantially more genetic information18–21. Specifically, after gap filling and probe circularization by a polymerase and ligase, the capture sequence can be amplified with the...
de Bitter, T.et al. Profiling of the metabolic transcriptome via single molecule molecular inversion probes.Sci Rep7, 11402,https://doi.org/10.1038/s41598-017-11035-0(2017). ArticleADSPubMedPubMed CentralGoogle Scholar Grundberg, I.et al.In situmutation detection and visualization of intratumor...
CTSB gene, including exon–intron boundaries (± 50bps) and the 5’ and 3’ untranslated regions (UTRs) using molecular inversion probes (MIPs) as described previously [35]. The full protocol is available at https://github.com/gan-orlab/MIP_protocol...
13 μl of Buffer A and 1.25 μl of Gap Fill Enzyme mix were added to each reaction and 9 μl of reaction volume was transferred to each of two wells in a 96-well plate. Molecular inversion probes were circularized with 4 μl of dNTP mix at 58°C for 10 min. Linear probes and ...
(UTRs) using molecular inversion probes (MIPs) as described previously [35]. The full protocol is available athttps://github.com/gan-orlab/MIP_protocol. Library sequencing was performed by the Genome Quebec Innovation Centre on the Illumina NovaSeq 6000 SP PE100 platform. We used Genome ...
A plurality of molecular inversion probes are tiled across a portion of the template. The probes are designed such that immediately adjacent probes hybridize to opposite strands of the nucleic acid template and probes on the same strand hybridize to the template in an overlapping manner. A region...