The molecular inversion probe (MIP) assay technology was originally developed for single nucleotide polymorphism (SNP) genotyping, but has subsequently been used for identifying other types of genetic variation including focal insertions and deletions, larger copy number alterations (CNAs), loss of ...
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et al. Highly multiplexed molecular inversion probe genotyping: over 10,000 targeted SNPs genotyped in a single tube assay. Genome Res. 15, 269–275, doi: 10.1101/gr.3185605 (2005). 18. Akhras, M. S. et al. Connector inversion probe technology: a powerful one-primer multiplex DNA ...
-grade astrocytomas, including 8 anaplastic astrocytomas [World Health Organization (WHO) grade III] and 27 glioblastomas (WHO grade IV) by immunohistochemistry, multiplex ligation probe-dependent amplification (MLPA), pyrosequencing of glioma-associated genes and molecular inversion probe (MIP) assay....
(SNPs) impeding hybridisation of the BNA probe and the padlock probe. Improvement of sensitivity of the method is necessary to detect co-expression of different transcripts in one cancer cell. However, the high specificity of the padlock assay will allow the investigation of intratumoural, ...
Oligonucleotide ligation assay ONT: Oxford nanopore technologies ORF: Open reading frame PAGE: Polyacrylamide gel electrophoresis paired-end RPLs: paired-end reduced representation libraries PAV: Presence/absence variations PCR: Polymerase chain reaction PCR-RFLP: Polymerase chain reaction-restriction...
Molecular Inversion Probe (MIP) Copy Number The MIP copy number assay was done as described previously [53] with some modifications. Specifically, the current protocol is a modification of the Targeted Genotyping protocol commercialized by Affymetrix [54]. Test DNA samples were diluted to16 ng/μ...
Molecular Inversion Probe (MIP) Copy Number The MIP copy number assay was done as described previously [53] with some modifications. Specifically, the current protocol is a modification of the Targeted Genotyping protocol commercialized by Affymetrix [54]. Test DNA samples were diluted to16 ng/μ...
Approximately 80 ng genomic DNA was utilized for molecular inversion probe array analysis. Genomic analysis was performed using the OncoS- can FFPE Assay kit (Affymetrix, Santa Clara, CA, USA), using a modified protocol.14 Briefly, genomic DNA is hybridized to a series of specialized nucleic ...
Hardenbol, 2005, Highly multiplexed molecular inversion probe genotyping: over 10,000 targeted SNPs genotyped in a single tube assay, Genome Res 15:269-75. Hards, 2006, Defects can increase the melting temperature of DNA-nanoparticle assemblies, J Phys Chem B 110 (33):16393-6. Harris, 20...