SP, REG, MJVMM and YJH performed the field activities with the collected samples. RB, SP, MMM, AM, PMB, YU, NOG, NAM, and HA performed samples treatment and the molecular analyses of the samples. RB, RA, and JCDA analysed the data. RB drafted the manuscript. JCDA, RA, HA, YU, ...
Intracellular reactive species production was evaluated in live cells by using 2’,7’-dihydrodichlorofluorescein diacetate (DCF-DA; Molecular Probes), according to the manufacturer. Briefly, cultures were washed with Krebs solution and incubated with 5 µM DCF-DA for 1 hour. In some experiments...
www.nature.com/scientificreports OPEN Molecular hydrogen regulates gene expression by modifying the free radical chain reaction- received: 13 July 2015 dependent generation of oxidizedaccepted:02December2015 phospholipid mediatorsPublished:07January2016 Katsuya Iuchi1,*, Akemi Imoto1,*,†, Naomi ...
Ratiometric and Near㊣nfrared Molecular Probes for the Detection and Imaging of Zinc Ionsanalytical chemistry, reviewluminescence, fluorescence (organic substances)organo﹝inc compoundsanalytical methodsChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted ...
b, Whole chromosome painting results with probes tiling chromosome 6 (green) and chromosome 12 (red) in single Jκ5-single Igh v-Abl cells (“Parental”, left) and Igh-Igκ hybrid v-Abl cells (“Translocation”, right). After translocation, a chr12-chr6 fusion chromosome is detected ...
Molecular Probes to Be Bought In a Deal for $325 Million.Reports on the plans of Carlsbad, California-based biotechnology company Invitrogen Corp. to acquire Molecular Probes Inc. for $325 million.EBSCO_bspWall Street Journal Eastern Edition...
The tissues were then washed with PBST (PBS–Tween 20) throughout the day, and the following probes were applied: donkey anti-rabbit Alexa Fluor 555, donkey anti-mouse Alexa Fluor 633, Oregon Green phalloidin 488, and DAPI. After an overnight incubation, all sections were wash...
Accordingly, the future directions of ST are to improve the spatial resolution, gene coverage, sensitivity, and reducing complexity of existing methods. We could develop new probes, arrays, sequencing strategies and imaging systems to increase the accuracy and throughput of ST. We also need to deve...
This approach can simultaneously detect hundreds of RNA molecules and allows the detection of up to 40 different fluorescent probes in a single cell [19, 20]. While ISH technologies offer high-resolution imaging, they require multiple rounds of hybridization and imaging, increasing experimental time ...
To date, there is still an absence of small-molecule probes or drugs that specifically inhibit DHHC-PATs. Among various lipid-based covalent inhibitors that paninhibit DHHC-PATs, 2-bromopalmitate (2-BP) is the most widely used and irreversibly inhibits S-palmitoylation by occupying the lipid bind...