Introduce directed mutation at specific positions of the MMLV RT sequence and codon optimization is needed to achieve these properties. The two RT coding sequences with (rRT-K) and without directed mutations (rRT-L) were versatility optimized and expressed to analyze the ribonuclease H (RNase H)...
In template switching–based scRNA-Seq, a known adaptor sequence fused to an oligo(dT) serves as a primer to initiate the reverse transcription at the 3′-end of an mRNA. As the reverse transcription reaction progresses, the RT is able to switch from the 5′-end of the mRNA template to...
• Hot Start Thermostable RT activates at temperatures consistent with the melting of RNA secondary structures. • Hot Start Thermostable RT can distinguish between a single base mismatch with a ΔCq of 6-8. (RNA sequences that differ at only 1 or 2 positions. 5 | Life Technologie...
Described are protein constructs of Moloney murine leukemia virus reverse transcriptase (MMLV-RT). In one embodiment the protein construct comprises a Moloney murine leukemia virus reverse transcriptase (MMLV) mature protein sequence; and an expression enhancement sequence at the N-terminus of said MMLV...
The protocol consists of reverse transcription of mRNA using M-MLVReverse Transcriptase, RNase H Minus, Point Mutant (Cat.# M3682, M3683) and Random Hexamers (Cat.# C1181), followed by PCR amplification of internal sequences of cDNA using 10mer primers with arbitrary...