The functionality of Moloney murine leukemia virus reverse transcriptase (MMLV RT) will increase with the improvement of its solubility and thermal stability. Introduce directed mutation at specific positions of the MMLV RT sequence and codon optimization is needed to achieve these properties. The two ...
nontemplated addition of three protruding nucleotides (+CCC) to the 3′-end of the nascent cDNA, which can then anneal to the matching rGrGrG 3′-end of the template-switching oligo (TSO), allowing the reverse transcriptase (RT) to switch templates and continue copying the TSO sequence. In...
In one embodiment the protein construct comprises a Moloney murine leukemia virus reverse transcriptase (MMLV) mature protein sequence; and an expression enhancement sequence at the N-terminus of said MMLV mature protein sequence. The expression enhancement sequence is devised for providing heterologous ...
The protocol consists of reverse transcription of mRNA using M-MLVReverse Transcriptase, RNase H Minus, Point Mutant (Cat.# M3682, M3683) and Random Hexamers (Cat.# C1181), followed by PCR amplification of internal sequences of cDNA using 10mer primers with arbitrary...