Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) is an RNA-dependent DNA polymerase that can be used for cDNA synthesis and subsequent PCR or qPCR in one-step or two-step RT-PCR or RT-qPCR assays.
2. Tanese, N. and Goff, S.P. (1988) Domain structure of the Moloney murine leukemia virus reverse transcriptase: mutational analysis and separate expression of the DNA polymerase and RNase H activities. Proc. Natl. Acad. Sci. USA 85, 1777–81. ...
The functionality of Moloney murine leukemia virus reverse transcriptase (MMLV RT) will increase with the improvement of its solubility and thermal stability. Introduce directed mutation at specific positions of the MMLV RT sequence and codon optimization is needed to achieve these properties. The two ...
We carried out the template-switching reverse transcription by using a 5′-FAM–labeled DNA primer and a TSO that contained three terminal guanosine ribonucleotides at the 3′-end (rGrGrG-3′ TSO) (Fig. 1A). As it has been reported that MMLV-based reverse transcriptases add multiple non...