We previously found that chi-miR-99b-3p was highly expressed in the skeletal muscle of 7-month-old (rapid growth period) goats and speculated that it may be associated with muscle development. To further investigate the role of chi-miR-99b-3p in goats, we found that chi-miR-99b-3p ac...
MiR-99b-5p/203a-3p overexpression reduced GC cell proliferation and cell cycle progressionin vitro. Notably, we combined bioinformatics tools with biological validation assays to demonstrate that insulin-like growth factor 1 receptor (IGF-1R) is a direct co-target and functional mediator of miR-99...
MicroRNAs (miRNAs) have been explored in many critical cellular processes, including proliferation and apoptosis. The purpose of this study was to detect the biological function and regulation of miR-99b-5p and miR-203a-3p in gastric cancer (GC). Here, we demonstrated that miR-99b-5p/203a-3...
我们研究发现,在脓毒症的病理情况下,血浆外泌体miR-1-3p表达升高,循环至肺组织时可被血管内皮细胞摄取,通过其靶基因SERP1抑制血管内皮细胞增殖,促进其凋亡和细胞骨架收缩,增加单层内皮细胞通透性和细胞膜损伤,损害内皮细胞功能削弱其...
An increase in both the expression of miR-140-3p and miR-99b-5p was able to identified patients with MDS and NK with good prognosis independently of IPSS-R risk category, improving the identification of patients with good outcomes (ROC curve for IPSS-R: 0,669 and for miRNAs model: 0,...
miR-135b-5p和miR-499a-3p的特异性引物,通过Real-Time PCR检测两种miRNA的表达.3,荧光素酶报告载体的构建:利用PCR技术扩增包含结合位点的MEF2C3'UTR区域,并将该区域连入荧光素酶报告载体,利用双荧光素酶报告试剂盒检测荧光强度的变化.4,Western blot:在HEK293和HUVEC细胞中分别转染miR-135b-5p和miR-499a-3p...
Ciliogenesis requires the removal of CP110 from the mother centriole; actin dynamics also influence ciliation, at least partly by affecting the centrosomal accumulation of ciliogenic membrane vesicles. How these distinct processes are properly regulated remains unknown. Here we show that miR-129-3p,...
The aim of the present study was to investigate the biological role and underlying mechanisms of action of miR-518a-3p in the progression and invasion of colorectal cancer (CRC). Reverse transcription-quantitative PCR (RT-qPCR) was used to examine the mRNA expression levels of miR-518a-3p in...