MCE 国际站:hsa-miR-99a-5p antagomir 产品活性:hsa-miR-99a-5p antagomir 是一种经过特殊化学修饰的成熟miRNA的互补单链,全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA antagomir通过特异性结合成熟miRNA,阻止miRNA与其靶基因的互补配对,从而抑制miRNA...
3.川芎嗪对脂多糖诱导的细胞屏障功能损伤的保护作用:实验结果显示,川芎嗪能够显著减轻脂多糖对HUVECs屏障功能的损伤,这一作用可能与上调miR-99a-5p及抑制mTOR信号通路有关。四、讨论本研究发现,川芎嗪能够通过上调miR-99a-5p的表达,进而抑制mTOR信号通路,从而减轻脂多糖对HUVECs屏障功能的损伤。这一机制可能为川芎嗪在...
In addition, cellular experiments including CCK8, wound healing, Transwell and flow cytometry assays disclosed that transfection of miR-99a-5p mimic could suppress the cell activity, cell migratory, and invasive abilities, and promote cell apoptosis, thus inhibiting the tumor progression of CSCC cells...
mmu-miR-99a-5p qPCR试剂盒 分类:microRNA qPCR Kits 品牌:BioTNT 货号:mmu-miR-99a-5p 原价:￥2730 现价:￥2100.00 节省:￥630 规格:1 kits 说明:mmu-miR-99a-5p 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方...
18.(9分)结核病是由结核分枝杆菌(MTB)感染引起的慢性传染性疾病 TB感染宿主后主要寄生在巨噬细胞内,感染后的噬细胞miR-99a-5p表达水平显著降低。科研人员利用转基因技术获得的miR-99a-5p过表达巨噬细胞作为实验组进行MTB感染实验,研究miR-99a-5p在此过程中的免疫调控作用,结果如下表感染24h组别TNF-a相表达IFN-...
miR-99a-5p, known to play an important role in mammalian target of rapamycin (mTOR) regulation, is downregulated in human bladder cancer. The study aimed to investigate the anticancer activity of miR-99a-5p and the possible mechanism associated with mTOR in bladder cancer cells. Vectors expressin...
中miR-99a-5p和miR-639的表达情况进行检测.然后利用实时定量逆转录PCR(Real-time PCR)技术在52例初诊MDS患者(包括31例IPSS低危和中危1在内的相对低危组患者,21例IPSS中危2和高危在内的相对高危组患者),18例初诊MDS转急性髓系白血病(以下简称"转白")患者及19例健康对照组的骨髓细胞中miR-99a-5p和miR-639的...
Shilin District, Taipei City 11101, Taiwan, Republic of China Tel +886 2 2833 2211 ext 2065 Fax +886 2 2838 9404 Email thomashwang0828@gmail.com Introduction: miR-99a-5p, known to play an important role in mammalian target of rapamycin (mTOR) regulation, is downregulated in human bladder ...
A total of 113 genes were identified as targets of pre-miR-99a regulation (19 genes modulated by miR-99a-5p, and 95 genes regulated by miR-99a-3p) in BrCa cells. Notably, FAM64A was targeted by both of the miRNAs. Among these targets, high expression of 16 genes (C5orf22, YOD1, ...
双荧光素酶报告实验结果显示,miR-99a-5p可以靶向FZD5的3'UTR区抑制荧光素酶报告基因的活性.以上实验说明miR-99a-5p可以通过调控FZD5发挥功能.(3)为了探究miR-99a-5p靶基因FZD5对类固醇激素分泌的影响,本研究首先通过免疫组化揭示了FZD5在山羊卵巢GCs的分布情况;然后利用FZD5干扰慢病毒,干扰GCs细胞中FZD5表达水平...