Mouse Mir99b qPCR Primer Pair,即小鼠Mir99b qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR)...
bta-miR-99b qPCR试剂盒 分类:microRNA qPCR Kits 品牌:BioTNT 货号:bta-miR-99b 原价:￥4030 现价:￥3098.38 节省:￥932 规格:1 kits 说明:bta-miR-99b 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶...
本发明公开了一种miR‑99b‑5p过表达的工程化间充质干细胞源外泌体的制备方法及其应用,间充质干细胞外泌体可运载miR‑99b‑5p特异性归巢到肿瘤部位,靶向结直肠癌细胞,进而在体内和体外抑制结直肠癌细胞的生长和转移。因而,miR‑99b‑5p过表达的工程化间充质干细胞源外泌体具有抑制结直肠癌恶性进展...
CCK-8法检测过表达miR-99b的SGC-7901细胞的增殖情况,Transwell法检测SGC-7901细胞系的迁移情况,Western blotting检测SGC-7901细胞系中哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)的表达情况.结果:miR-99b在胃腺癌细胞系中的表达水平显著低于胃上皮细胞系,其中以SCG-7901细胞最甚.转染miR-99b模...
Mir99b microRNA 99b [Mus musculus (house mouse)] 来自 NCBI 喜欢 0 阅读量: 37 作者: entrezgene 摘要: microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability ...
MicroRNAs (miRNAs) have been explored in many critical cellular processes, including proliferation and apoptosis. The purpose of this study was to detect the biological function and regulation of miR-99b-5p and miR-203a-3p in gastric cancer (GC). Here, w
inresponsetoTGF-b.However,mir-99aandmir-99binhibitionwasinsufficienttoblockTGF-binducedEMTofNMUMG cells.Mir-99aandmir-99bover-expressioninepithelialNMUMGcellsresultedinincreasedproliferation,migrationand fibronectinexpression,whileE-cadherinandZO-1expressionwerenegativelyregulated.Inconclusion,weidentifiedmir- 99a...
Notably, we combined bioinformatics tools with biological validation assays to demonstrate that insulin-like growth factor 1 receptor (IGF-1R) is a direct co-target and functional mediator of miR-99b-5p/203a-3p in GC cells. Mechanistically, the AKT pathway, which is downstream of IGF-1R, is...
本发明公开了一种miR99b5p过表达的工程化间充质干细胞源外泌体的制备方法及其应用,间充质干细胞外泌体可运载miR99b5p特异性归巢到肿瘤部位,靶向结直肠癌细胞,进而在体内和体外抑制结直肠癌细胞的生长和转移.因而,miR99b5p过表达的工程化间充质干细胞源外泌体具有抑制结直肠癌恶性进展的作用.为探索利用外泌...
目的:探讨上调microRNA-99b(miR-99b)表达对宫颈癌HeLa细胞增殖和凋亡的作用及可能的机制。 Objective:To investigate the effects of microRNA-99b(miR-99b)up-regulated on proliferation an...