1.一种miR-489-3p在制备诊断和治疗人骨质疏松症药物中的应用,其特征在于包括以下步骤: (1)设计miR-489-3p特异性引物序列,并合成制备miR-489-3p特异性引物; (2)miR-489-3p特异性引物应用:收集骨质疏松患者骨组织样本、建立老龄小鼠骨质疏松模型、后肢去负荷骨质疏松小鼠模型检测miR-489-3p在骨组织中表达量,证实...
表达显著下调,细胞增殖能力显著降低(P<0.05);转染miR-489-3p inhibitor可以显著降低PC12细胞的miR-489-3p水平,而BDNF的蛋白表达显著上调,并且显著提高了细胞的增殖能力(P<0.05).过表达或抑制miR-489-3p对BDNF的mRNA表达并无显著影响.结论:miR-489-3p可以在转录后水平靶向负调控BDNF的表达.miR-489-3p抑制PC12...
couldbindmiR-489-3pandSOX4whichmightbethetargetgeneo miR-489-3p.ResultsWe rst oundthatSNHG5wasup-regulatedinbothAMLpatientbonemarrowsamplesandvariousAMLcelllines.Second,we oundthatknockdowno SNHG5inhibitedproli erationo AMLcellsandpromotedapoptosis.Itwas oundthatSNHG5couldbindmiR-489-3p,andthe...
sion. Mechanistically, LINC01446 sponged miR-489-3p function, which leaded to TPT1 overexpression. In all, this study demon- strates a critical role of LINC01446/miR-489-3p/TPT1 singling pathway in regulating GBM progression. 2. Materials and methods 2.1. Human samples 31 pairs of GBM sampl...
Through luciferase activity assay, RNA pull-down, and RNA-binding protein immunoprecipitation (RIP), we proved that SNHG5 could bind miR-489-3p and SOX4 which might be the target gene of miR-489-3p.ResultsWe first found that SNHG5 was up-regulated in both AML patient bone marrow samples...
apoptosisbrain-derived neurotrophic factorglioblastomainvasionmigrationmiR-489-3pAmong astrocyte tumors, glioblastoma (GBM) is the most malignant glioma, highly aggressive and invasive, with extremely poor prognosis. Previous research has reported that microRNAs (miRNAs) participate in the progression of many...
目的miRNA通过调节相应靶基因和各种信号通路的表达,参与肺癌的产生与扩散,miR-489-3p可作为靶向治疗靶点,并且已被证实在多种肿瘤中起抑癌作用,微小染色体维持蛋白(MCM)异常表达与肿瘤细胞增殖,侵袭,转移密切相关.本文miR-489-3p通过调控MCM2影响肺癌细胞的增殖.方 法本课题将以从中国科学院上海细胞库购买了肺癌细胞...
Our study suggested that thisrepeat sequence could sequester multiple miR-489-3p and inhibit bone formationthrough miR-489-3p/SMAD2 axis. Moreover, siRNA of Lnc-DIF would rescue bone formation in both aging and ovariectomized osteoporosis mice. This study revealed a kind of LncRNA that could ...
Upregulation of miR-489-3p and miR-630 inhibits oxaliplatin uptake in renal cell carcinoma by targeting OCT2 OCT2mi RNARenal cell carcinomaEpigenetic regulationOxaliplatinRenal cell carcinoma(RCC) is one of the most common malignant tumors affecting the urogenital system, accounting for 90% of ...
检测miR-489-3p和MCM2 mRNA的表达水平;CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡;Western印迹检测蛋白表达;双荧光素酶报告基因检测实验检测细胞的荧光活性.结果相较于正常肺上皮细胞BEAS-2B,肺癌细胞A549,H1299,SPC-A1中miR-489-3p表达水平均显著降低,MCM2 mRNA和蛋白表达水平显著升高(P<0.05).miR-489-3p过...