在非小细胞肺癌中,miR-424-5p的表达水平与肿瘤的恶性程度、转移能力等密切相关。然而,其具体作用机制尚不明确。因此,研究miR-424-5p在非小细胞肺癌中的作用及调节机制,对于揭示肺癌发病机制、诊断及治疗具有重要意义。 三、研究方法 1.实验材料:非小细胞肺癌细胞A549、miR-424-5p模拟物及抑制剂、相关试剂等。 2...
因此,研究miR-424-5p在非小细胞肺癌细胞A549中的作用及调节机制,对于深入了解肺癌的发病机制及开发新的治疗方法具有重要意义。 二、研究方法 本研究采用实时荧光定量PCR技术检测非小细胞肺癌细胞A549中miR-424-5p的表达水平,通过细胞增殖、凋亡及迁移实验探究miR-424-5p对A549细胞生物学行为的影响。同时,利用生物信息...
miR-424-5p过表达对乳腺癌细胞增殖 和迁移能力的影响doi:10.3969/j.issn.1002-266X.2019.19.012李自涛马志军刘震沈国双Shandong Medical Journal
The targeting relationship between miR-424-5p and CHEK1 was validated through a dual-luciferase reporter assay. Furthermore, the effects of miR-424-5p on HCC cell proliferation, migration, and invasion were evaluated using the Cell Counting Kit-8 assay, wound healing assay, and Transwell ...
The effect and mechanism of miR-424-5p on the invasion and migration of human pancreatic cancer is unknown. Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of miR-424-5p and CYLD in pancreatic cancer specimens and pancreatic cancer cells PANC-1. MiR-424-5p mimics...
P <0.05)㊂miR -424-5p 是lncRNA NEAT1在调节ox -LDL 诱导的HUVEC 损伤中的功能介质,ELK4是miR -424-5p 的直接靶标(P <0.05)㊂miR -424-5p 的抑制或ELK4的过表达逆转了lncRNA NEAT1沉默对ox -LDL 诱导的HUVEC 细胞损伤的影响(P <0.05)㊂结论:沉默lncRNA NEAT1可通过调控miR -424-5p/...
中发挥抑癌基因的作用,过表达miR-424-5p能够抑制宫颈癌细胞的克隆,侵袭,并促进细胞凋亡;miR-424-5p可能通过GPR30作用于PI3K/AKT/mTOR通路从而抑制宫颈癌的恶性... 王佳佳,丁佳,常永霞,... - 《山东医药》 被引量: 0发表: 2021年 hsa-miRNA-424-5p靶向GPR30对宫颈癌恶性生物学行为的影响 中发挥抑癌基因...
The correlation of LATS1 and has-miR-424-5p (miR-424) expression with clinicopathological characteristics and prognosis of GC patients was analyzed by TCGA RNA-sequencing data. A novel circular RNA_LARP4 (circLARP4) was identified to sponge miR-424 by circRNA expression profile and bioinformatic...
MiR-424-5p has been reported to participate in various tumors proliferation and metastasis as a suppressor. On the contrary, miR-424-5p would promote cell proliferation in some tumors. However, the expression of miR-424-5p in intrahepatic cholangiocarcinoma (ICC) is rarely reported and its ...
International Journal of Molecular Sciences Review Sequence Requirements for miR-424-5p Regulating and Function in Cancers Jiangying Xuan 1,2,† , Yingxia Liu 1,†, Xiaoping Zeng 1 and Hongmei Wang 1,* 1 School of Basic Medical Sciences, Nanchang University, Nanchang 330006, China; xjy...