circ_0001785在CRC组织及细胞系中呈高表达, 而miR-330-5p呈低表达, 二者呈负相关, 其表达量与分化程度、淋巴结转移及肿瘤分期密切相关; 体外细胞实验结果显示干扰circ_0001785表达可抑制细胞增殖、迁移及侵袭; circ_0001785可靶向结合miR-330-5p; circ_0001785可通过靶向调控miR-330-5p而发挥作用. 实验结论 干扰ci...
方法:体外培养人心肌细胞AC16,转染miR-330-5p模拟剂(mimic),在1%O2+5%CO_(2)+94%N_(2)条件下构建心肌细胞氧糖剥夺/复氧(OGD/R)模型,以模拟心肌缺血再灌注。采用流式细胞技术检测细胞凋亡;采用ELISA法检测心肌细胞中4-羟基壬烯醛(4-HNE)、丙二醛(MDA)的含量;采用WesternBlot法检测凋亡相关因子Bcl-2相关的...
miR-330-5p的靶基因,miR-330-5p负调控Nox4表达.抑制miR-330-5p表达或过表达Nox4可促进Cyclin D1和Bcl-2蛋白表达(t=9.664,7.548),抑制P21和Bax蛋白表达(t=10.184,10.314),增强心肌细胞增殖活力(t=6.667~9.126),抑制细胞凋亡(t=10.341),差异具有统计学意义(均P<0.01).抑制Nox4表达能够逆转抑制miR-...
CIRC 0008532在膀胱癌组织和细胞系中的表达显著上调,并通过海绵化miR-155-5p/miR-330-5p与膀胱癌的进展呈正相关,从而影响膀胱癌组织中MTGR 1的表达和Notch信号的活性。 Circ_0008532可能发挥调节作用,成为膀胱癌治疗的靶点。 2 循...
三级亚组血清miR-330-5p,miR-34a-5p,VEGF水平依次升高(F/P=54.260/0.000,161.507/0.000,11.309/0.000);Pearson分析结果显示,急性缺血性脑卒中患者血清miR-330-5p,miR-34a-5p与VEGF水平均呈正相关(r/P=0.674/0.000,0.624/0.000);ROC分析结果显示,血清miR-330-5p,miR-34a-5p,VEGF水平及三者联合预测急性缺血性...
摘要: 目的:探讨环状RNA 0010729(circ_0010729)是否靶向调控miR-330-5p影响白细胞介素(IL-1β)诱导的软骨细胞损伤.方法:实时定量PCR(RT-qPCR)分析IL-1β诱导后软骨细胞中circ_0010729和miR-330-5p表达量.双荧光素酶报告实验和RT-qPCR确定circ_0010729对miR-330-5p的靶向调控... 查看全部>>...
www.nature.com/cddiscovery ARTICLE OPEN Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p Jianye Zhong 1,4, Abai Xu1,2,4, Peng Xu1, Minhong Su3, Peng Wang1, Zhe Liu1, Boping Li1, Chunxiao Liu 1 ✉ and Ning Jiang 2 ✉ ...
miR-330-5pTRAF6NF-κBLPS-induced sepsisCardiomyopathySepsis is a whole-body inflammation and main cause of death in intensive care units worldwide. We aimed to investigate the roles of lncRNA MIAT and miR-330-5p in modulating inflammatory responses and oxidative stress in lipopolysachariden (LPS...
However, the role of miR-330-5p in myocardial I/R injury-induced inflammatory responses is unknown. This study aimed to investigate the role of miR-330-5p in NLRP3 inflammasome-mediated myocardial I/R injury. Methods Myocardial I/R injury was induced in mice by occlusion of the left ...