该研究的目的是为了观察circEPSTI1是否通过靶向调控miR-216b-5p对胃癌细胞顺铂耐药性产生影响,并探讨其可能的作用机制.采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction, qRT-PCR)检测胃癌组织,癌旁组织,人胃黏膜细胞GES-1,人胃癌细胞HGC27与胃癌耐药性细胞HGC27/DDP中circEPSTI1与...
摘要 目的:探讨miR-216b-5p对食管癌Eca109细胞顺铂(DDP)耐药性的影响及其作用机制。方法:采用qPCR法检测miR-216b-5p在食管癌细胞TE-1、KYSE-150、Eca109和耐药细胞Eca109/D...展开更多 Objective:To investigate the effect of miR-216b-5p on cisplatin(DDP)resistance in esophageal cancer Eca109 cells and...
The regulatory effect of miR‐216b‐5p on either Linc00518 or GATA6 was interrogated with luciferase reporter assay. The endogenous GATA6 protein was analysed by Western blotting. The cell viability was measured by MTT assay and IC50 of paclitaxel was calculated through cell counting. Results: ...
TU686细胞系目的检测miR-216b-5p对喉癌细胞系TU686的增殖,迁移,侵袭和凋亡的作用,探求其在喉癌细胞中的靶基因以验证其可能的作用机制.方法将喉癌TU686细胞分为两组,实验组通过慢病毒感染过表达miR-216b-5p,对照组感染携带阴性对照序列的慢病毒,并利用实时荧光定量PCR(q RT-PCR)验证感染效率;使用CCK-8实验检测...
Conclusion:MiR-216b-5p can inhibit the proliferation, migration, and invasion and induce apoptosis of laryngeal cancer cells to exert a tumor suppressor effect, and its mechanism may be related to the targeted regulation of the expression level of ATG5.于宗男...
迁移侵袭目的探讨miR-216b-5p在卵巢癌细胞系A2780中的表达情况,及其对卵巢癌细胞增殖,迁移和侵袭能力的影响.方法采用实时荧光定量聚合酶链反应(real-time quantitative PCR),检测miR-216b-5p在正常卵巢上皮细胞系IOSE-80和卵巢癌细胞系A2780中的表达情况.在A2780中过表达及抑制miR-216b-5p后,采用CCK-8实验,...
miR-216b-5pTo characterize Linc00518 expression in prostate cancer and elucidate the potential mechanistic involvement in paclitaxel resistance. The relative expression of Linc00518 and miR-216b-5p was determined by real-time PCR. The regulatory effect of miR-216b-5p on either Linc00518 or GATA6...
And the control group had higher cell proliferation than miR-216b-5p mimic group (P<0.05). The miR-216b-5p inhibitor group had more cell metastasis and invasion than the miR-216b-5p mimic group and the control group (P<0.05). The miR-216b-5p mimic ...
miR-216b-5pSKILBackground Circular RNA 0029803 (circ_0029803) was found to be upregulated in colorectal cancer (CRC) tissues, but its function and underlying molecular mechanism are not studied in CRC. Methods The expression levels of circ_0029803, microRNA-216b-5p (miR-216b-5p), and ski...
miR-216b-5p目的探讨叉头框D1(FOXD1),miR-216b-5p在子宫内膜异位症(EMs)患者子宫内膜组织中的表达及其临床意义.方法选取2018年1月至2021年6月在我院就诊的188例EMs患者作为研究组,分别取EMs患者异位子宫内膜组织(异位组)以及在位子宫内膜组织(在位组),另选取在我院因子宫肌瘤进行手术的患者185例,取正常子宫...