目的:通过建立银屑病小鼠模型,观察麻防犀角地黄汤对银屑病皮损及miR-215-5p的影响,探讨麻防犀角地黄汤治疗银屑病的作用机制.方法:咪喹莫特(IMQ)乳膏局部涂抹诱导建立银屑病小鼠模型,麻防犀角地黄汤灌胃干预.观察小鼠体质量,PASI评分,左耳耳廓外缘的厚度,皮损组织的病理改变;qRT-PCR法检测皮损中miR-215-5p的表达...
货号:tgu-miR-215-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:tgu-miR-215-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转...
货号:aca-miR-215-5p 原价:￥4030 现价:￥3098.38节省:￥932 规格:1 kits 说明:aca-miR-215-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录产...
目的:通过建立银屑病小鼠模型,观察麻防犀角地黄汤对银屑病皮损及miR-215-5p的影响,探讨麻防犀角地黄汤治疗银屑病的作用机制.方法:咪喹莫特(IMQ)乳膏局部涂抹诱导建立银屑病小鼠模型,麻防犀角地黄汤灌胃干预.观察小鼠体质量,PASI评分,左耳耳廓外缘的厚度,皮损组织的病理改变;qRT-PCR法检测皮损中miR-215-5p的表达水平...
However, the relationship between miR-215-5p and Se deficiency in myocardial development remains unknown. In this study, CCCTC-binding factor (CTCF) was confirmed as the target gene of miR-215-5p by dual luciferase reporter assay, western blot and quantitative real-time polymerase chain reaction ...
本课题组前期研究发现,IAV可以引起肺上皮细胞源外泌体miRNA差异表达,通过外泌体miR-1249-5p靶向调节溶质载体家族4成员A1(solute carrier family 4 member 1,SLC4A1)基因抑制核因子-κB(nuclear factor-κB,NF-κB)信号通路,从而参与...
与此不同,miR-215-5p inhibitors转染前脂肪细胞后,在增殖和分化上获得与miR-215-5p mimics转染后相反的结果.同时,采用双荧光素酶报告基因系统,验证了 mi-R-215-5p 的靶基因,过表达miR-215-5p可抑制核受体辅激活因子3(nuclear receptor coactivator 3,NCOA3)基因3'-UTR区荧光活性,而突变NCOA3基因3'-UTR区与...
目的:通过建立银屑病小鼠模型,观察麻防犀角地黄汤对银屑病皮损及miR-215-5p的影响,探讨麻防犀角地黄汤治疗银屑病的作用机制.方法:咪喹莫特(IMQ)乳膏局部涂抹诱导建立银屑病小鼠模型,麻防犀角地黄汤灌胃干预.观察小鼠体质量,PASI评分,左耳耳廓外缘的厚度,皮损组织的病理改变;qRT-PCR法检测皮损中miR-215-5p的表达...
miR-215-5pYY1MelanomaObject To investigate the role of circHIPK3 in melanoma. Methods Bioinformatics analysis and experiments including RT-qPCR, Pearson's correlation analysis, luciferase reporter, Western blot, and RIP assays were applied to explore the function and mechanism of circHIPK3 in ...
In this study, we report that miR-215-5p can promote myocardial autophagy by directly binding to the 3'untranslated region (3'UTR) of phosphatidylinositol-4, 5-bisphosphate 3-kinase (PI3K). Its target gene PI3K was confirmed by dual luciferase reporter assay, quantitative real-time polymerase ...