第二部分mir-138-1*对afb1所致的b-2a13细胞恶性转化的影响及调控机制目的:探讨mir-138-1*对afb1所致的b-2a13细胞的恶性转化过程的影响,分析mir-138-1*调控的分子机制。方法:采用瞬时转染的方法改变mir-138-1*或pdk1在p50b-2a13细胞的表达水平。用体外研究评价mir-138-1*或pdk1对p50b-2a13细胞的增殖、迁移、...
In present study, 36 upregulated and 27 downregulated miRNAs in P50 B-2A13 cells were first identified by miRNA microarray, and miR-138-1* was selected as a candidate miRNA by RT-qPCR and pilot experiments. Functional studies revealed that miR-138-1* could inhibit proliferation, colony ...
近日,来自中国医科大学第一医院泌尿外科朱育焱团队在J Exp Clin Cancer Res杂志上发表了题为“HES1-mediated down-regulation of miR-138 sustains NOTCH1 activation and promotes proliferation and invasion in renal cell carcinoma”的文章,该研究表明miR-138-2是一个新的致癌NOTCH1途径的表观遗传调节因子,由HES1...
MiR-138 indirectly regulates the MDR1 promoter by NF- k B/p65silencingJ.L. Requenez-Contrerasa , b , E.S. L ?opez-Castillejosa , b , R. Hern ?andez-Floresa , b ,M.A. Moreno-Eutimioc , J.T. Granados-Riveron a , G.U. Martinez-Ruiz d ,G. Aquino-Jarquina , *a Laborato...
miR-138-5p/SIRT1/FOXO1 轴在体外抑制成骨分化:添加 miR-138-5p 抑制剂后,FOXO1 的 mRNA 和蛋白水平显著增强,而 SIRT1 shRNA 和 FOXO1 shRNA 则使其表达减弱。同时,miR-138-5p 抑制剂处理后,OCN、OPN、RUNX2 和 ALP 的蛋白水平升高,添加 shRNA SIRT1 或 FOXO1 后则降低,矿化结节数量变化也符合这一趋...
Furthermore, miR-138-1-3p affected the stability of the CRIPTO-GRP78 complex on the cell membrane and also reversed the radioresistant characteristics of NPC stem cells, which affected EMT and the JAK2/STAT3 signaling pathway. Conclusions: The miR-138-1-3p is a small molecule that can ...
用miR-138-5p抑制剂转染心肌细胞后,miR-138-5p的表达受到抑制(图5a,P <0.05),而KLF3-AS1和Sirt1的表达上调(图5b-d, P <0.05)。在心肌细胞中转染KLF3-AS1过表达载体会导致KLF3-AS1(图5e,P <0.05)和Sirt1(图5g,h,P <0.05)的上调以及miR-138-5p的下调(图5e,P <0.05) 5f,P <0.05)。miR-138-...
miR-138 mimic对GRC-1细胞HIF-1α mRNA水平的影响, 采用Western blot检测miR-138对GRC-1细胞HIF-1α蛋白水平的影响.分别采用CCK-8,流式细胞术和划痕实验检测miR-138-5p靶向HIF-1α对GRC-1细胞的增殖,凋亡和迁移的影响.结果荧光素酶报告实验结果显示miR-138-5p可靶向HIF-1α.与GRC-1组比较, miR-138 ...
The expression of miR-138 (A) and SIRT1 (B) in hippocampus of mice induced by CUMS were determined by RT-PCR. (C) The correlation of miR-138 mRNA level and SIRT1 expression. *** p < 0.001. Display full size Figure 2 miR-138 increased depression-like behavior in CUMS models ...
胶质母细胞瘤(Glioblastoma multiforme,GBM)是最常见的恶性程度最高的成人颅内肿瘤,临床预后极差。目前人们对胶质瘤的发生发展的生物学机制仍不清楚,而且其诊断和预后缺乏特异性的生物标记物。大量研究表明EZH2对胶质瘤细胞周期与增殖发挥关键调控作用。我们通过临床胶质瘤样品标本及TCGA数据库,验证了miR-138在GBM中显著...