The aims of this study were to elucidate the basic role of betatrophin and miR-122a in metabolic syndrome and cancer development related metabolic perturbation. Here, we have separated our study into human case-control study and animal model by using the miR-122a-/- mice. On clinical ...
产品编号:600728查看说明书 产品名称:miR-122a .0订购此产品 供应商:Agilent 规格: 目录价:￥:2179.52 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址:点击查看详细 打印此页关闭此页 上一个:miR-107[600727 /] 下一个:miR-126[600729 /]...
1.生物信息学分析发现miR.122a的靶基因涉及细胞周期、信号转导、细胞增殖、 分化和细胞凋亡等众多生物学过程。 2.过表达miR.122a可增加肝癌细胞的放疗敏感性,其具体机制可能通过下调直接 作用靶基INcyclinG1,使细胞周期阻滞于G2/M期,这种调节作用与p53基因表达状态 ...
Methods are provided for the treatment of cardiovascular or metabolic diseases characterized by elevated serum total cholesterol, elevated serum LDL-cholesterol, or elevated serum trigylcerides, through the administration of an oligomeric compound which modulates the levels or activity of miR-122a. ...
为了进一步研究miR-122a对肝癌细胞放疗敏感性的作用以及分子机制,我们从预测的miR-122a靶基因中筛选出与放射敏感性密切相关的两个分子cyclin G1和APE1进行分析.cyclin G1是新近发现的一种细胞周期蛋白,在多种肿瘤中高表达,其基因启动子内包括两个p53蛋白的结合位点,是"p53-细胞周期"信号转导通路的重要效应分子,...
目的运用实时荧光定量RT-PCR方法分析miR-122与miR-224两种miRNAs在原发性肝细胞癌(HCC)中的表达,探讨以miRNAs为靶点在早期肝癌中的临床诊断意义。方法采用基于2(-△ACT)的实时荧光定量RT.PCR法对35例肝癌组织及癌旁组织的miR-122a及miR-224进行了定量分析,结果经由Northern blot验证。结果与正常组织相比,在所有的...
目的:研究miR-122a对人喉癌细胞株Hep2细胞增殖,细胞周期以及相关蛋白表达量的影响.方法:人喉癌细胞株Hep2细胞分别转染miR-122a寡聚核苷酸(A组)和miR-122a inhibitor(阻遏物)(B组),同时设立阻遏物阴性对照(inhibitor negative control,miR-NC inhibitor)(C组)和空白对照(D组).用RT-PCR,MTT法,流式细胞仪和...
Conclusion: The decreased levels of peripheral blood miR-122a and miR-146a of patients with AD are closely related to the severity of the disease, and which may participate in the occurrence and development of AD by regulating the Th1/Th2/Th17 immune ...
Methods are provided for the treatment of cardiovascular or metabolic diseases characterized by elevated serum total cholesterol, elevated serum LDL-cholesterol, or elevated serum trigylcerides, through the administration of an oligomeric compound which modulates the levels or activity of miR-122a. ...
miR-122aHepG2凋亡bcl-2p53目的:探究过表达miR-122 a是否可提高肝癌细胞HepG2对化疗药物的敏感性,并阐明其提高化疗药物敏感性的部分机制.方法:构建过表达miR-122 a的HepG2细胞系(miR-122a肿瘤细胞组),并与HepG2细胞(肿瘤细胞组)置于含不同浓度顺铂的培养液中,CKK-8法观察miR-122a上调对HepG2细胞存活率...