miR-144 and miR-451 are encoded by a single miRNA locus and their mature sequences, only 100 bp apart, are on the same primary RNA [16]. The miR-451 expression is largely restricted to red blood cells, though a low level of miR-451 is observed in non-erythroid lineages. Remarkably...
The protective effects of Rg1 were abolished by injecting of AAV-HIF-miR-144-shRNA into the predicted ischemic penumbra. In conclusion, our results demonstrate that Rg1 alleviates oxidative stress after I/R through inhibiting miR-144 activity and subsequently promoting the Nrf2/ARE pathway at the...
有文献报道shRNA靶向沉默ZEB2表达通过Wnt/β-catenin信号通路降低肺癌细胞增殖活性[23]。本研究表明下调HOXA5激活了A431细胞内的JAK/STAT信号通路,且通过该通路促进癌细胞的增殖、侵袭和EMT;提示miR-196-5p靶向HOXA5可能通过JAK/STAT信号通...
Flow cytometric assays revealed that knockdown of CCDC144NL-AS1 induced SGC7901 and BGC823 cells apoptosis (Fig. 3a–c). Subsequently, we inoculated subcutaneously the SGC7901 cells stably transfected with shRNA targeting CCDC144NL-AS1 or their negative control groups into female nude mice to ...
The shortcomings is that we have not inhibited the mRNA of HGF (by siRNA/shRNA) to identify the same phenotype as the overexpression of miR-144-3p, which might be detected in our future research. These observations support the proposal that miR-144-3p takes part in lung cancer development ...
shRNA靶向沉默ZEB2表达通过Wnt/β catenin信号通路降低肺癌细胞增殖活性的实验研究[J]. 现代肿瘤医学, 2019, 27(4):541 546. 基金 河南省科技发展计划项目(202102310397) PDF(1887 KB) 617 Accesses 0 Citation Detail 段落导航 相关文章 摘要 Abstract 关键词 Key words 引用本文 参考文献 基金...
The control vector (pMXs) contains a scramble shRNA sequence. Retroviral particles, obtained by transfecting these vectors into Phoenix A packaging cells, were used to infect Huh-7 cells that were selected by adding puromycin (10 µg/ml) 48 h after infection. HCC cell lines were transiently...
lncRNA can regulate tumorigenesis development and distant metastasis of colorectal cancer (CRC). However, the detailed molecular mechanisms are still largely unknown. Using RNA-sequencing data, RT-qPCR, and FISH assay, we found that HIF1A-AS2 was upregul
Detection and Quantitation of siRNA Production from Transduced shRNA Expression Constructs The QuantiMir™ cDNAs were synthesized from Human T-Rex 293 cells transduced with equivalent amounts of Lentivirus containing the SBI shRNA expression constructs for the p53 shRNA driven by the H1 promoter ...
NC: negative control; sh-ST8SIA6-AS1: shRNA-ST8SIA6-AS1. Full size image ST8SIA6-AS1 sponged miR-142-3p in LIHC cells Binding site sequences of ST8SIA6-AS1 on miR-142-3p were obtained using StarBase (Fig. 5A). The luciferase assay revealed that the luciferase activity of cells...