When transposon- containing cells are selected on rich medium, insertions occur at both attTn7 sites with equal efficiency and frequency. Because carA mutants are arginine and pyrimidine auxotrophs, single- site insertions at the glmS attTn7 sites can be obtained by selection on minimal medium. ...
Large-scale mutagenesis of the yeast genome using a Tn7-derived multipurpose transposon. We present here an unbiased and extremely versatile insertional library of yeast genomic DNA generated by in vitro mutagenesis with a multipurpose element ... Kumar,A. - 《Genome Research》 被引量: 94发表:...
When transposon-containing cells are selected on rich medium, insertions occur at both attTn7 sites with equal efficiency and frequency. Because carA mutants are arginine and pyrimidine auxotrophs, single-site insertions at the glmS attTn7 sites can be obtained by selection on minimal medium. From...