When transposon-containing cells are selected on rich medium, insertions occur at both attTn7 sites with equal efficiency and frequency. Because carA mutants are arginine and pyrimidine auxotrophs, single-site insertions at the glmS attTn7 sites can be obtained by selection on minimal medium. From...
Large-scale mutagenesis of the yeast genome using a Tn7-derived multipurpose transposon. We present here an unbiased and extremely versatile insertional library of yeast genomic DNA generated by in vitro mutagenesis with a multipurpose element ... Kumar,A. - 《Genome Research》 被引量: 94发表:...
3)Mini-Tn10 transposon systemmini-Tn10转座系统 英文短句/例句 1.Using Mini-Tn10 Transposon System to Research the Genes Involved in Biofilm Formation in Bacillus利用mini-Tn10转座系统研究芽孢杆菌生物被膜形成相关基因 2.Application of Geographic Information System Technology in Mini VR-GIS;地理信息系统...
Mini-Tn 10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed...
a转座质粒pIC333是针对芽孢杆菌而构建的转座插入载体,其中含有来自于鼠伤寒沙门氏菌Mini-Tn10转座子,除此以外,该质粒还具有壮观霉素抗性基因、红霉素抗性基因 Swivel material particle pIC333 is aims at the swivel insertion carrier which the bud spore bacillus constructs, in which includes comes from in mo...
marcescens transposon mutant library, we found that mutants defective in the biosynthesis of LPS O-antigen and flagella, cell surface components required for bacterial motility, exhibit attenuated hemocyte-killing ability (42). This LPS- and flagella-dependent hemocyte killing by S. marcescens impairs ...
亚抑制浓度的四环素(0.2mg / L)加速了从粪便E.粪便到肉毒小鼠肠道中单核细胞增多性李斯特菌的接合转座子Tn1545转移频率10倍,并且这不是由于对接合子的选择性作用。体内结果证实了体外结果,其中通过滤膜接种方法观察到由于抗菌处理而增加20倍。86 在一项实验中获得了对转移疗效影响的间接证据,其中临床相关水平的四环素...
extorquens using the mini-Tn7 transposon system. Cultures containing the chromosomally integrated cymR gene were subsequently transformed with pCHOI3 containing... YJ Choi,L Morel,D Bourque,... - 《Applied & Environmental Microbiology》 被引量: 197发表: 2006年 Vitamins/minerals and genomic stabili...
mirabilis by transformation, followed by selection of insertion- containing strains; third, PCR verification of mini- Tn7 insertions; and last, optional Flp- mediated excision of the antibiotic- resistance selection marker present on the chromosomally integrated mini- Tn7 element. When transposon- ...