Microplate Enzyme Assay Using FluorescenceVotano, JrParham, MHall, Lh
Combining the sensitivity of fluorescence-based assays and convenience of microplate format allows researchers to achieve rapid and quantitative readout using high-throughput analysis. Within a microplate well, the fluorescent signal can be generated with intact cells, cell lysates, or purified enzyme pre...
Assay type Fluorescent Use Recommended in cases of extended viability studies or when using a high cell density in microplate assays Recommended for quick viability determination in microplate assays (10-minute incubation) Measurement Detecting metabolic activity of mammalian cells, bacter...
Microplate readers read biological, chemical or physical events that occur on a microtiter plate. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and scre...
(e.g., dot blots), enzyme activity assays (e.g., reverse transcriptase and kinases), receptor binding assays, and cell proliferation assays. A common feature of all these assays is that a labelled complex must be separated from any excess tracer that does not react with the target ...
More than 30 predefined assay protocols Quick-start button to get started quickly Real-time data display during measurement Endpoint and kinetic detection Sequential and simultaneous multi-detection Spectral scanning (absorbance, fluorescence, luminescence) One-click top/bottom reading switch Multiple well-...
with an absorbance microplate spectrophotometer you will be able to measure enzyme activities,quantity of DNAand proteins, cell viability and many more. However, you are limited to assays that might have lower sensitivity or specificity than applications employing other detection modes. If you currently...
Besides continually adjustable bandwidths up to 100 nm, BMG LABTECH’s Advanced LVF Monochromators allow for highly sensitive emission scanning in both fluorescence and luminescence assays. Using the broadest bandwidth of 100 nm, significantly lower concentrations can be achiev...
To determine performance of the IMAP kinase assay with red fluorophore, another Lck kinase dilution curve was performed using the TAMRA-p34cdc2- derived peptide (Figure 5). Here, an enzyme concentration range of 0.04 to 3.5 units/mL yielded a delta mP of 258, with a Z...
enzyme-based screens or antibiotic screening programs. For both fungal and bacterial colonies there are also marked differences in gene expression of the various areas of the colonies during development that could be monitored over time using this technique and evaluated depending on the study context...