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Marek, M., Günther-Pomorski, T.Assay of Flippase Activity in Proteoliposomes Using Fluorescent Lipid Derivatives.Methods Mol. Biol.1377, 181-191 (2016). Coleman, J. A., Kwok, M. C. M., Molday, R. S.Localization purification, and functional reconstitution of the P4-ATPase Atp8a2, a ...
专利名称：FLUORESCENCE-BASED ASSAY FOR THE DETECTION OF SPECIFIC NUCLEIC ACIDS USING PHOTON COUNTING 发明人：DOWBEN, ROBERT, M.,MARLIN, ALFORD, L.申请号：US0221353 申请日：20020703 公开号：WO03004686A3 公开日：20030821 专利内容由知识产权出版社提供 摘要：The present assay is directed to a ...
Connolly et al., Determination of the number of endothelial cells in culture using an acid phsophatase assay, Anal. Biochem. 152:136-140 (1986). Crouch et al., The use of ATP bioluminescence as a mesure of cell proliferation and cytotoxicity, J. Immunol. Meth. 160: 81-88 (1993). ...
said microdevice comprising a body comprising a poly(cyclic olefin), said body having an ozone-activated surface substantially free of intrinsic fluorophores; wherein said activated surface is substantially free of intrinsic fluorescence during a fluorescence-based assay of said biomolecules bound to said...
Here we describe a sensitive fluorescence-based assay to monitor bacterial apolipoprotein N-acyltransferase activity.Similar content being viewed by others Fluorescence anisotropy assays for high throughput screening of compounds binding to lipid II, PBP1b, FtsW and MurJ Article Open access 14 April ...
Amsbio's Screen Quest Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilisation across a broad spectrum of biological targets. Calcium signaling is a key event in many physiological processes, but has also been linked to pathways that are ...
Fluorescence-based thermal shift assays Thermal shift assay ThermoFluor® Differential scanning fluorimetry (DFS) Carrie Partch Dept. o..
Real-time fluorescence-based assay for HCV NS5bΔ21 and FMDV 3D polymerases Polymerase activity assays with HCV NS5bΔ21 were performed as described above for ZIKV RdRp. For the detection of FMDV 3D polymerase activity, 49 μL of reaction mix [50 mM Tris-HCl, pH 7.5, 0.6 mM MnCl2...
Fluorescence-based Thermal Shift Assay The fluorescence-based thermal shift assay is a recently adapted means to perform affinity screening based on a well-known theory. Theory behind the assay: Ligand binding to a target protein can stabilize a protein's native state, as shown in the increase ...