产品名称 :Complex I Enzyme Activity Microplate Assay试剂盒 参阅全部 :Complex I 试剂盒 检测方法: Colorimetric 样品类型 :Cell culture extracts, Tissue 检测类型 :Enzyme activity 实验步骤 :Multiple steps
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The activity of mitochondria complexes was evaluated in isolated mitochondria using the Complex I Enzyme Activity Microplate Assay Kit (ab109721, Abcam, Cambridge, MA) and the Mitochondrial Complex III Activity Assay Kit (MAK360, MilliporeSigma, St. Louis, MO) according to the manufactures’ instruc...
assaycomplex Icomplex IIIinsectmicroplatemitochondriaNADHKinetic microplate-based assays for both mitochondrial NADH:ubiquinone oxidoreductase (complex I) and succinate:cytochrome oxidoreductase using insect submitochondrial particles as the source of the enzyme activities have been developed. These assays have ...
The activities of Complex I and Complex IV were measured using the Complex I Enzyme Activity Microplate Assay Kit or the Complex IV Human Duplexing Microplate Assay Kit, both from MitoSciences, according to the manufacturer's instructions. Mitochondrial Membrane Potential Mitochondrial membrane potential...
In-gel aconitase activity assays were performed as described previously [46]. The NADH dehydrogenase activity of isolated mitochondria was measured using a complex I enzyme activity microplate assay kit (ab109721, Abcam, Shanghai, China). The complex II assay was performed with the Succinate-coenzym...
complex I activity driving reverse electron transport and the production of reactive oxygen species. Mechanistically, blocking complex I in pro-inflammatory microglia protects the central nervous system against neurotoxic damage and improves functional outcomes in an animal disease model in vivo. Complex I...
Measurement of the enzymatic activities of aconitase and complex I/II In-gel aconitase activity assays were performed as described previously [46]. The NADH dehydrogenase activity of isolated mitochondria was measured using a complex I enzyme activity microplate assay kit (ab109721, Abcam, ...
complex I enzyme activity assay kit (Abcam) was used. In brief, proteins extracted from the isolated mitochondria were loaded onto a microwell plate and complex I proteins were attached to the microplate via antibody coupling. Complex I activity was measured by the optical density (OD450nm) afte...
a color reaction corresponding to the activity of an enzyme if conjugated to a primary antibody can be effected by adding a chromogenic substance to the enzyme, and the color reaction corresponds to the amount of the antibody, namely the amount of the antigen present on the tissue. However, ...