We also show that the Siglech gene locus is a feasible site for specific targeting of microglia in the nervous system. In conclusion, Siglec-H is a reliable marker for microglia that will allow histological identification of microglia and microglia-specific gene manipulation in the nervous system....
Here, we present a novel reporter mouse model targeting a microglia-specific marker (TMEM119) for studying the function of microglia in health and disease. By placing a reporter cassette (GSG-3xFlag-P2A-tdTomato) between the coding sequence of exon 2 and 3'UTR of the Tmem119 gene using ...
2F). To confirm that this effect was due to SELP knockdown, we treated GB-microglia spheroids with a SELP specific small-molecule inhibitor (SELPi). SELPi treatment of GB cells in the presence of microglia resulted in a similar reduction in cell invasion (Fig. 2F), indicating that SELP ...
This study provides a direct comparison of metabolism between mouse and human microglia, highlighting the species-specific pathways involved in immunometabolism and the importance of considering these differences in translational research.Similar content being viewed by others Mitochondrial complex I activity ...
Selective permeability to molecules based on their molecular weight and lipid solubility Presence of specific markers Rich in two enzymes: gamma glutamyl transpeptidase (GGTP) (for amino acid transport) and alkaline phosphatase (transport of phsopahte ions) used as marker for BBB. Others: glucose ...
Cells expressing ionized calcium binding adapter molecule 1 (Iba1), a specific marker of microglia/macrophages, increased in number in the virally infected region of the trigeminal ganglion, with morphological transformation to an activated phenotype, frequently detected as perineural satellites. Further ...
大鼠游离特异性抗原(fPSA)试剂盒Rat Free Prostate Specific Aigen,fPSA ELISA Kit钴粉(>99%,BR) Cobaltous powder质量规格:>99%,BR 英文名称HumanCD26ELISAKit人CD26规格:96T/48T铜粉(>99.5%,BR) Copper powder质量规格:>99.5%,BR 二(DPA)DNA比色定量测定试剂盒10碱式碳酸铜(铜度:52.5~56.5%) Copper(II)...
Tmem119, a microglia-specific marker. Cdc20, a monocyte-specific marker. Error bars: standard error. cpm, counts per million. (E) Diagrams showing switches in the major poly(A) sites at the Ubxn4 and Araf loci (top row, “RefSeq”) in activated microglia as identified by cTag-PAPERCLIP...
2a–b). Accordingly, only microglia from d3 post-depleted mice were proliferating as determined by PCNA+ immunohistochemistry and these proliferating clusters were confirmed to be microglia as they were all TMEM119+, a marker microglia-specific marker10,22 (Supplementary Fig. 2c, d). We also ...
Presumably but not exclusively LPL, a marker of the disease-specific microglia51, was expressed in microglia whose activation was mediated by PQBP1 (Supplementary Fig. 8). TREM2 signaling also leads to NFκB activation83 and our data revealed that deficiency of PQBP1-cGAS–STING pathway leads ...