ProT2® MHC Class II Tetramers may be used to study CD4+ T cell immune responses in infectious diseases such as CMV (see figure 1 above) and HCV, and in vaccine studies. ProT2® staining for CD4+ T cell epitopes from HCV
MHCTetramer技术:检测抗原特异性T细胞的金标准 MHC 四聚体(MHC Tetramer)是一类免疫学试剂,由四个主要组织相容性复合体(Major Histocompatibility Complex,MHC)与抗原肽结合的单体分子组成,并标记有荧光,用于 T 细胞免疫分析的 MHC 四聚体检测(MHC Tetramer Assay,或称 MHC Tetramer Stain)。 检测原理 & 应用领域 M...
In situ tetramer staining (ISTMHC class I tetramersCD8+ T cellsvibratomeconfocal microscopyWith the onset of MHC tetramer technology came a wealth of new understanding of antigen specific CD8 + T cells. This chapter discusses the application of MHC tetramer technology to stain antigen specific T ...
・QuickSwitch™ Custom Tetramer Kits >MHC Tetramer【Custom】 ・MHC Class I custom Tetramer ・MHC Class II custom Tetramer >Tips for better staining ・How to use? ・Staining method ・Induction of specific CTL Examples of use Catalog search ...
Non-Invasive: MHC-peptide tetramer staining is a non-invasive technique, it relies on flow cytometry or fluorescence microscopy to identify T cells binding to the tetramer. Quantification: This technique allows quantification of T cells, providing an accurate measure of the frequency of antigen-specif...
J Clin Invest. 112(6): 831-42. [PubMedID: 12975468]. [4] Scriba et al. (2005). Ultrasensitive detection and phenotyping of CD4+ T cells with optimized HLA class II tetramers staining. J Immunol. 175(10): 6334-43. [PubMedID: 16272285].分享到: ...
参考 [1] Novak et al. (1999). MHC class II tetramers identify peptide-specific human CD4(+) T cells proliferating in response to influenza A antigen. J Clin Invest. 104(12): R63-7. [PubMedID: 10606632].
Here, using a combination of in situ functional assays, yeast display/MHC-I tetramer staining, and a range of complementary biophysical techniques including solution NMR, we address the G24-R36 loop discrepancy between the two MHC-I/TAPBPR X-ray structures16,17 and the MHC-I/Tapasin cryoEM...
γ or MHC class I tetramer staining, the breadth of these responses was greater in patients with progressive HIV infection compared with the LTNP group... SA Migueles,M Connors - 《Immunology Letters》 被引量: 134发表: 2001年 Examination of CD8+ T Cell Function in Humans Using MHC Class...
Newell, E.W., Klein, L.O., Yu, W. & Davis, M.M. Simultaneous detection of many T-cell specificities using combinatorial tetramer staining.Nat. Methods6, 497–499 (2009). ArticleCASGoogle Scholar Newell, E.W. et al. Combinatorial tetramer staining and mass cytometry analysis facilitate T...