We therefore characterised a novel antibody specific for MICA/B which binds a wide range of MICA alleles at a unique epitope in the α3 domain. Using this antibody we demonstrate for the first time that MICA/B is more broadly expressed across a wide range of normal tissue epithelia, and co...
S. Involvement of clathrin and AP-2 in the trafficking of MHC class II molecules to antigen-processing compartments. Proc. Natl Acad. Sci. USA 102, 7910–7915 (2005). Article CAS PubMed PubMed Central Google Scholar Santambrogio, L. et al. Involvement of caspase-cleaved and intact ...
. For the detection of MHC class I, cells were also stained with FITC-labeled goat anti-mouse secondary antibody (555,988, BD Pharmingen). Results were analyzed using FlowJo software (Tree Star). Enzyme-linked immunosorbent assay (ELISA) The supernatant fluid of the cocultured system was ...
The classical major histocompatibility complex class I (MHCI) molecules are key players in initiating an immune response against intracellular pathogens such as viruses. The mature classical MHCI molecule is divided into three alpha domains where the two most distal domains are involved in peptide bindi...
IRGQ directs misfolded MHC class I toward lysosomal degradation through its binding mode to GABARAPL2 and LC3B. In the absence of IRGQ, free MHC class I heavy chains do not only accumulate in the cell but are also transported to the cell surface, thereby promoting an immune response. Mice...
Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 Basic information Product Name: Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 Synonyms: Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 CAS: MF: MW: 0 EINECS: Product Categories: Mol File: Mol File Anti-MH...
molecules (Inabaet al., 2000). However, the delivery of an inflammatory stimulus results in the loading of these MHC class II molecules with these antigens and the trafficking of these antigenic peptide—MHC class II complexes to the cell surface via CIIV (Inabaet al., 2000;Turleyet al.,...
The higher affinity of HLA-G for LILR1 and, consequently, ILT2, may result from the presence of phenylalanine and tyrosine in the positions 195 and 197 of the alpha-3 domain, respectively. This altered structured, when compared with other HLA class I molecules, increase hydrophobicity, which ...
Antigen presentation via major histocompatibility complex class I (MHC I) molecules is essential to mount an adaptive immune response against pathogens and cancerous cells. To this end, the transporter associated with antigen processing (TAP) delivers sn
S. Involvement of clathrin and AP-2 in the trafficking of MHC class II molecules to antigen-processing compartments. Proc. Natl Acad. Sci. USA 102, 7910–7915 (2005). Article CAS PubMed PubMed Central Google Scholar Santambrogio, L. et al. Involvement of caspase-cleaved and intact ...