MHC class 1 cytoplasmic domain : defining a role for conserved amino acid residues in class 1 expression, trafficking, and antigen presentation 来自 circle.ubc.ca 喜欢 0 阅读量: 25 作者: GA Lizee 摘要: MHC class I molecules contain within their cytoplasmic domains a tyrosine and two orthree ...
1f). These data demonstrate that TCR-MAP is a specific and sensitive system to capture cognate pMHC–TCR interactions for both class I-restricted (HLA-A2) and class II-restricted T cells in humans. TCR-MAP captures mouse MHC class I-restricted (H2-Kb) and class II-restricted (H2-IAb) ...
MHC class I surface expression on cells transfected with empty vector pcDNA3.1 was unaffected (dashed lines), while the population of RP9 cells reactive with the FLAG antibody (red line) showed a clear down-regulation of MHC class I on the surface (red arrow) of approximately 300 mean ...
FULL PAPER British Journal of Cancer (2017) 116, 1208–1217 | doi: 10.1038/bjc.2017.79 Keywords: MICA; MICB; immunohistochemistry; immunofluorescence; confocal microscopy; novel antibody; normal tissue expression; tumour tissue expression MHC class I chain-related protein A and B (MICA and MICB)...
. For the detection of MHC class I, cells were also stained with FITC-labeled goat anti-mouse secondary antibody (555,988, BD Pharmingen). Results were analyzed using FlowJo software (Tree Star). Enzyme-linked immunosorbent assay (ELISA) The supernatant fluid of the cocultured system was ...
molecules (Inabaet al., 2000). However, the delivery of an inflammatory stimulus results in the loading of these MHC class II molecules with these antigens and the trafficking of these antigenic peptide—MHC class II complexes to the cell surface via CIIV (Inabaet al., 2000;Turleyet al.,...
Major histocompatibility complex (MHC) class II molecules are required for the presentation of antigenic peptides that are derived predominantly from inter
Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 Basic information Product Name: Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 Synonyms: Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 CAS: MF: MW: 0 EINECS: Product Categories: Mol File: Mol File Anti-MH...
(MHC) class I restricted CD8+T cells was, indeed, enhanced by its being attached to the proposed translocatory sequence of the HIV-1 tat protein. However, we found little evidence that the increased immunogenicity resulted from transfer of the fusion peptide between living cells, and we ...
S. Involvement of clathrin and AP-2 in the trafficking of MHC class II molecules to antigen-processing compartments. Proc. Natl Acad. Sci. USA 102, 7910–7915 (2005). Article CAS PubMed PubMed Central Google Scholar Santambrogio, L. et al. Involvement of caspase-cleaved and intact ...