Protein traffickingPulse-chaseSecretory pathwayTo understand peptide selection by MHC class I molecules on a molecular level, the folding, assembly, and peptide binding of class I molecules have been intensely investigated in recent years. In contrast, the placement of these events into the cellular ...
T-cell activation requires many interactions with peptide–MHC complexes on antigen-presenting cells, such as dendritic cells (DCs). Two reports in Nature now show that DCs might be able to enhance the probability of productive interactions with T cells by the directed trafficking of peptide–MHC...
Major histocompatibility complex (MHC) class I chain-related protein A (MICA) and MHC class I chain-related protein B (MICB) are polymorphic proteins that are induced upon stress, damage or transformation of cells which act as a ‘kill me’ signal through the natural-killer group 2, member...
The SH3 domain-binding surface and an acidic motif in HIV-1 Nef regulate trafficking of class I MHC complexes Nef, a regulatory protein of human and simian immunodeficiency viruses, downregulates cell surface expression of both class I MHC and CD4 molecules in T ce... M.,E.,Greenberg - 《...
HIV-1 Nef-induced down-regulation involves endosomal trafficking and a cooperative interaction between the cytoplasmic domain (CD) of MHC-I, Nef, and the clathrin adaptor protein complex-1 (AP-1). The CD of MHC-I contains a key tyrosine within the sequence YSQA that is required for down-...
Misfolded MHC class I heavy chains (MHC I HCs) are targeted for endoplasmic reticulum (ER)-associated degradation (ERAD) by the ubiquitin E3 ligase HRD1, and E2 ubiquitin conjugating enzyme UBE2J1, and represent one of the few known endogenous ERAD substrates. The mechanism by which misfolded...
This pathway operates in TAP negative cells and is also blocked by agents that raise endosomal pH or inhibit vesicular trafficking 89, 114, 115, 116. The invariant chain, used for directing MHC class II traffic, has been shown to direct a subset of MHC class I molecules to the endocytic ...
PTC cell lines K1 and TPC-1 were stimulated with IL-17A and analyzed for MHC class I expression afterwards. Cluster of differentiation (CD) 8+T cell activation, production of Interleukin-2 (IL-2) and Interferon-gamma (IFN-γ) as well as the programmed death-1 (PD-1) expression on ...
Interferon-gamma mediates antigen trafficking to MHC class II-positive late endosomes of enterocytes. Eur Immunol. 2005;35(3):831–42.Buning J, Schmitz M, Repenning B, Ludwig D, Schmidt MA, Strobel S, Zimmer KP (2005) Interferon-gamma mediates antigen trafficking to MHC class II-positive ...
Antigen presentation via major histocompatibility complex class I (MHC I) molecules is essential to mount an adaptive immune response against pathogens and cancerous cells. To this end, the transporter associated with antigen processing (TAP) delivers snippets of the cellular proteome, resulting from ...