1999. Chromatographic methods to study protein-protein interactions. Methods 19:278-305.Beeckmans S. Chromatographic methods to study protein-protein interactions. Methods. 1999. 19; 278-305.S. Beeckmans. Chromatographic methods to study protein–protein interactions. Methods 19 :278–305 (1999). ...
Hence, it is of importance to study the RNA-protein interactions in vivo. Numerous methods have been developed to identify the target nucleotide sequences of RBPs. The methods include the electrophoretic mobility shift assay (EMSA), systematic evolution of ligands by exponential enrichment (SELEX),...
Proteins and enzymes are now generally thought to be organized within the cell to form clusters in a dynamic and versatile way, and heterologous protein–protein interactions are believed to be involved in virtually all cellular events. Therefore we need appropriate tools to detect and study such ...
1. Ramanathan M, Porter DF, Khavari PA, et al. Methods to study RNA-protein interactions. Nat Methods. 2019 Mar;16(3):225-234. doi: 10.1038/s41592-019-0330-1.
and those that examine RNAs bound to a protein of interest (protein-centric). Each method has unique strengths and limitations, which makes it important to select optimal approaches for the biological question being addressed. Here we review methods for the study of RNA-protein interactions, with...
这为研究circRNA与蛋白的相互作用提供了一定的研究思路。 参考文献 1. Ramanathan M, Porter DF, Khavari PA, et al. Methods to study RNA-protein interactions. Nat Methods. 2019 Mar;16(3):225-234. doi: 10.1038/s41592-019-0330-1.
Protocol for using Promega's HaloCHIP™ to characterize DNA-protein interactions. Image courtesy of Promega. For example Promega’s HaloCHIP™ System, which the company promotes as an alternative to ChIP, covalently captures intracellular DNA-protein complexes without using antibodies. Cells express...
This is mainly due to technical difficulties in their identification and in differentiating between interactors of circRNAs and their linear counterparts. Here we review the currently used methodology to systematically study circRNA-protein complexes (circRNPs), focusing either on a specific RNA or ...
These modern techniques allow the study of protein-protein interaction in intact cells, and can be used to monitor monomer association and dissociation in vivo. Recently, oligomerization has also been reported in the case of the TSH receptor (TSHR). This review will focus on the previously un...
审稿| 王建民参考资料Reed, T.J., Tyl, M.D., Tadych, A. et al. Tapioca: a platform for predicting de novo protein–protein interactions in dynamic contexts. Nat Methods 21, 488–500 (2024). https://doi.org/10.1038/s41592-024-02179-9...