Hence, it is of importance to study the RNA-protein interactions in vivo. Numerous methods have been developed to identify the target nucleotide sequences of RBPs. The methods include the electrophoretic mobility shift assay (EMSA), systematic evolution of ligands by exponential enrichment (SELEX),...
非编码 RNA 序列,包括长非编码 RNA、小核仁 RNA 和非翻译 mRNA 区域,通过与 RNA 结合蛋白 (RBP) 的直接相互作用来实现许多不同的功能。最近的工作已经鉴定出数百个缺乏已知RNA结合域的新RBP,从而强调了RNA-蛋白质复合物的复杂性和多样性。最近的进展扩大了研究 RNA-蛋白质相互作用的方法数量,主要分为两大类:...
研究人员可通过RNA-蛋白质交联鉴定体内两者的相互作用,原理是在变性情况下,排除非共价结合的相互作用,纯化RNA,接着只提取发生交联的蛋白以用于鉴定。 甲醛是一种小的双功能交联剂,可以很容易地渗透细胞并交联2Å距离内的大分子,包括蛋白质—蛋白质,蛋白质—DNA和蛋白质—RNA复合物,形成可逆的共价连接,导致很难区分...
研究人员可通过RNA-蛋白质交联鉴定体内两者的相互作用,原理是在变性情况下,排除非共价结合的相互作用,纯化RNA,接着只提取发生交联的蛋白以用于鉴定。 甲醛是一种小的双功能交联剂,可以很容易地渗透细胞并交联2Å距离内的大分子,包括蛋白质—蛋白质,蛋白质—DNA和蛋白质—RNA复合物,形成可逆的共价连接,导致很难区分...
[Methods in Molecular Biology] RNA-Protein Interaction Protocols Volume 488 || Immunoprecipitation Analysis to Study RNA-Protein Interactions in Xenopus Oocytes[Methods in Molecular Biology] RNA-Protein Interaction Protocols Volume 488...
Proteins and enzymes are now generally thought to be organized within the cell to form clusters in a dynamic and versatile way, and heterologous protein–protein interactions are believed to be involved in virtually all cellular events. Therefore we need appropriate tools to detect and study such ...
in vivoprofiling of protein-RNA interactions is subject to the transcript abundances in the experimental cell type. Thus, researchers must instead rely on computational methods to impute missing binding sites on non-expressed transcripts or to characterize RBP binding sites in settings where no ...
DNA-protein interactions (DPIs) are critical to all living organisms, particularly in the regulation of gene expression, replication, packing, recombination, and repair, as well as RNA transport and translation. Since microscopically observing interactions between proteins and DNA in the late nineteenth...
et al. Comparison of methods and resources for cell–cell communication inference from single-cell RNA-seq data. Nat. Commun. 13, 3224 (2022). This study introduces LIANA, a computational tool including multiple existing strategies to infer CCIs and distinct ligand–receptor resources. In addition...
a new mutant BirA* was engineered from Bacillus subtilis, termed BASU, that enables >1,000-fold faster kinetics and >30-fold increased signal-to-noise ratio over the prior standard Escherichia coli BirA*, thereby enabling direct study of RNA–protein interactions in living cells on a timescale...