Protein-protein interactions (PPIs) play key roles in various aspects of cellular regulation. Accordingly, PPI analysis is crucial for systems-level understanding of any biological process. This chapter describes two major methods for PPI analysis, namely, mass spectrometry (MS)-based approaches and ...
Golemis E (2002)Protein-protein interactions: A molecular cloning manual.Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press. p ix, 682. Phizicky EM, Fields S (1995) Protein-protein interactions: Metho...
蛋白质的相互作用(Protein-ProteinInteractions).docx,第十一章 蛋白质的相互作用(Protein-Protein Interactions) 概况 随着人类基因组测序工作的完成,生命科学进入到后基因组和蛋白组的时代。因此,蛋白质的相互作用争论就显得越来越重要。生命活动过程与蛋白质的相互作用
4. Methods Used to Investigate Protein–Ligand Binding Affinity 4.1. Experimental Methods 4.2. Theoretical/Computational Methods 5. Conclusions Abstract 分子识别是生物大分子与其他生物大分子或各种小分子以高特异性和亲和力形成特定复合物的过程,构成了生物体内所有过程的基础。蛋白质是一类重要的生物大分子,通过与...
Determining protein partners is an essential step toward understanding protein function and identifying relevant biological pathways. Many methods exist for investigating protein–protein interactions. The pull-down assay is an in vitro technique used to detect physical interactions between two or more prot...
第十一章蛋白质的相互作用(Protein-ProteinInteractions) 1.概况 随着人类基因组测序工作的完成,生命科学进入到后基因组和蛋白组的时代。因此,蛋白质的相互作用研究就显得越来越重要。生命活动过程与蛋白质的相互作用是密不可分的,如DNA合成、基因转录激活、蛋白质翻译、细胞周期调控、信号转导等重要的生命过程均涉及到...
Experimental and computational studies are paving way for a deeper understanding of the dynamic nature of protein–protein interactions. Arunima Singh Comments & Opinion06 Dec 2024Nature Methods Volume: 21, P: 2226-2227 Three decades of protein-fragment complementation ...
30. Computational Prediction of Protein–Protein Interactions John Obenauer; Michael Yaffe pp. 445-468 31. Affinity Methods for Phosphorylation-Dependent Interactions Greg Moorhead; Carol MacKintosh pp. 469-478 32. Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes ...
1. Such interactions vary from being permanent to transient2,3. Some protein–protein interactions are specific for a pair of proteins, while some proteins are promiscuous and interact with many partners. This complexity of interactions is a challenge both for experimental and computational methods....
蛋白质相互作用(protein interaction)的研究方法 蛋白质相互作用的研究方法 万维松 2016-7-23