The present invention relates to the isolation, characterization and / or identification of microorganisms in the test sample. 本发明的方法包括用于溶解可能存在于测试样品中的非微生物细胞的可选的溶胞步骤,接着是后续的分离步骤. The method of the present invention comprises means for dissolving the test...
Methods are described for the isolation of intact intestinal epithelium in several novel forms. The first method described is for application to segments of bowel that must be studied without killing the animal, e.g., a resected length of bowel. In this method, the isolated segment is everted...
Method-specific biases in the composition of microbial taxa. Another major source of biases in metagenomic analysis is the varied efficiency of DNA purification for different microbial groups. Given the same staring material, different DNA isolation methods will result in vastly different quantities and ...
A new method for the isolation of intracellular and extacellular DNA from a range of sediment types has been developed. This method is based upon the direc... A Ogram,GS Sayler,T Barkay - 《Journal of Microbiological Methods》 被引量: 1285发表: 1987年 Detection and enumeration of bacteria...
of plant-microorganism interactions, infiltration is used for the controlled introduction of bacterial suspensions into leaf tissues or for the isolation of microorganisms inhabiting apoplastic spaces of leaves. The methods based on infiltration of target tissues allow the penetration of dyes, fixatives ...
申请(专利权)人: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM 发明人:JE Becvar,Laura Becvar 被引量: 22 摘要: Methods for the isolation and identification of a toxicant in a sample are disclosed. Luminescent biological agents (i.e., bacteria) having sensitivity to a toxicant or an ...
Large-bore pipets大口径移液管:reduce shearing during transfer&limit the number of transfers Table 1. Isolation of Genomic DNA I 1. Harvest cells with method appropriate to sample type 2. If necessary wash cells free from media by resuspension in cold phosphate-buffered saline and centrifugation ...
Standard concentrations of antimicrobial inhibitors (e.g., 0.05% sodium azide) can be added to prevent growth of cellulolytic microorganisms if the membranes are stored wet for long periods of time before use. Membranes should not be...
In certain embodiments, the methods of the invention provide for the concentration or isolation of microorganisms from a sample, such as, e.g., a matrix, as described herein, thereby facilitating detection of the microorganisms via nucleic acid amplification. The methods and kits of the present ...
微生物学英文教学课件:chapter2 The Methods for Studying Microorganisms.ppt,The scanning electron microscope provides some of the most dramatic and realistic images in existence. This instrument is designed to create an extremely detailed three-dimensiona