clinical microbiology and clinical chemistry comparative evaluation of selective and nonselective culture techniques for isolation of group a beta-hemolytic streptococci 来自 dx.doi.org 喜欢 0 阅读量: 20 作者:DF Welch,D Hensel,D Pickett,S Johnson,B ...
The unexpected epimerization of Leu units in the lajollamide framework observed during our studies clearly demonstrates the danger in misassigning natural product structures elucidated by spectroscopic methods and relatively harsh analytical techniques alone. This demonstrates the importance of total synthesis...
In Theory and Practice of Histological Techniques, 7th ed.; Suvarna, S.K., Layton, C., Bancroft, J.D., Eds.; Churchill Livingstone Elsevier: London, UK, 2013; pp. 179–220. [Google Scholar] Heuer, H.; Krsek, M.; Baker, P.; Smalla, K.; Wellington, E.M. Analysis of ...
Recent advancements have been made in high-throughput sequencing, which can provide sequencing depths to cover various bacterial ecosystems, e.g., soil, sediment, animal, insect gut, marine or freshwater [20–24]. The combination of high-throughput sequencing and culture-dependent techniques was ...
Since IL-based extraction techniques have the capability of isolating and enriching various organic and inorganic compounds, we implemented them in our brain sample preparation method development. We also identified the most appropriate medium for the homogenization of rat brain slices and the ...
Microfluidic or chip-based cell sorters have great potential for single cell isolation in microbiology and biotechnology because they can be used for a variety of sample sizes, including small, precious samples of few cells, or large samples which include interesting rare cells. They can offer ...
Complementary DNA (cDNA) was synthesized using 1 µg of RNA with Omniscript RT kit (Qiagen, Hilden, Germany), followed by quantitative real-time PCR techniques. The qPCR reaction had a final volume of 20 µL, where 5 µL of cDNA was added to 15 µL of a PCR mix containing 10...
In order to complete a double evaluation of possible differences using either microbiological or molecular techniques, we then accepted the introduction of possible alterations of the original bacterial communities due to the different cultural media and incubation cycles. Moreover, this approach avoided ...
Bacillus cereus strains were obtained from the Guangdong Institute of Microbiology and stored at −40 ◦C in 25% (v/v) glycerol. 2.2. Isolation and Purification of DLc1 The phage vB_BceP-DLc1 was isolated from the sewage samples collected in the Huangsha aquatic product market (Guangzhou...
Techniques such as transcriptomics and metabolomics, which are used to reveal expressed genes and associated metabolites, are now being applied as tools in the discovery and expression of natural products. 'Omics'-based natural product discovery pipelines are still in their infancy, but have shown ...